促红细胞生成素对全脑缺血再灌注损伤大鼠血红素加氧酶-1表达的影响  

Effects of erythropoietin on heme oxygenase-1 expression in rats with globle ischemia-reperfusion injury

作　　者：黄涛[1] 林晓英[2] 李芳邻[1] 钟宁[1] 刘运芳[1] 孟晓慧[1] 郑玉兰[1] 宋文延[1] 

机构地区：[1]山东大学医学院诊断学研究所,济南250012 [2]济南市中心医院神经内科,济南250013

出　　处：《山东大学学报（医学版）》2008年第3期225-228,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨促红细胞生成素(EPO)对大鼠脑缺血再灌注后血红素加氧酶1(HO-1)表达的影响。方法随机将雄性SD大鼠分为缺血再灌注组、治疗组、假手术组。治疗组按照缺血再灌注后不同时间点给予EPO腹腔注射,假手术组不手术,于各时间点断头取脑,用逆转录-聚合酶链反应技术检测大鼠皮层HO-1 mRNA的表达。结果①全脑缺血再灌注后HO-1 mRNA的表达发生动态变化,缺血后6h开始明显升高,24 h达高峰,此后缓慢下降;②EPO能够显著上调全脑缺血后皮层HO-1 mRNA的表达,缺血再灌注后即注射EPO组与缺血再灌注组各时间点HO-1和-βactin吸光度的比值差异有统计学意义(P<0.05)。结论EPO的神经保护作用可能部分通过上调HO-1的表达来实现。Objective To explore the effect of erythropoietin（EPO） treatment on the expression of heme oxygenase-1 （HO-1 ）mRNA in rats with globe ischemia-reperfusion injury, Methods The 4-vessel obstructive globe ischemic rat models were used in this study. The rots were randomly divided into three groups： the ischemia-reperfusion group, the experimental group and sham-operation group. Rats in the ischemia-reperfusion group and the experimental group were subjected to EPO injection into the abdominal cavity after globe ischemia, however rots in the sham-operation group were not subjected to an operation. The expression of HO- 1 mRNA was determined by RT-PCR. Results （1） After ischemia, the expression of HO-1 mRMA was obviously changed based on time. （2） The effect of EPO treatment on the expression of HO- 1 mRNA in the cortex of this rat model was obvious. Conclusion The protective effect of EPO for ischemia-reperfusion injury is perhaps induced by up-regulating the expression of HO-1.

关 键 词：血红素氧化酶(脱环) 红细胞生成素 再灌注损伤 

分 类 号：R743.33[医药卫生—神经病学与精神病学]