血小板膜糖蛋白Ibα基因HPA-2、Kozak序列多态性与脑梗死的相关性研究  

Relationship between the HPA-2 and Kozak sequence polymorphism of platelet membrane glycoprotein and cerebral infarction

作　　者：郑敏[1] 郝跃伟[1] 刘雪平[1] 赵婷婷[1] 

机构地区：[1]山东大学附属省立医院老年神经科,济南250021

出　　处：《山东大学学报（医学版）》2008年第3期292-295,共4页Journal of Shandong University:Health Sciences

基　　金：山东省科学技术发展计划项目资助课题(2002BB1DBA6)

摘　　要：目的探讨血小板膜糖蛋白(platelet membrane glycoprotein,GP)Ibα基因HPA-2、Kozak序列多态性与脑梗死的相关性。方法选取316例经CT/MRI证实的脑梗死患者做病例组,209例与病例组年龄、性别相匹配的无脑血管病人群做对照组。采用聚合酶链反应-限制性片断长度多态性(polymerase chain reactionrestriction fragment lengthpolymorphism,PCR-RFLP)技术检测人类血小板抗原-2(human platelet antigen-2,HPA-2)及Kozak序列多态性在两组中的分布频率。结果病例组GPIbα基因HPA-2等位基因频率、基因型与对照组相比差异无统计学意义(P>0.05)。病例组Kozak序列C等位基因频率为25.95%,对照组C等位基因频率为18.18%,两组间差异具有统计学意义(χ2=8.132 3,P<0.01)。结论GPIbα基因HPA-2序列多态性与脑梗死无相关性;Kozak序列多态性可能是脑梗死的遗传易感因素。Objective To explore the relationship between polymorphism of platelet membrane glycoprotein I bα HPA-2 and Kozak sequence and cerebral infarction in the Chinese. Methods Three hundred and sixteen patients with cerebral infarction confmned by CT/MRI and two hundred and nine controls were genotyped for the HPA-2 and Kozak polymorphism by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis. Results There were no significant differences in the HPA-2 genotype frequencies and allele frequencies between cerebra vascular thrombosis patients and controls. The Kozak genotypes with the C allele were 25.95% in the cerebral infarction group compared with those of the control group（18.18%）. Statistical signs showed significant differences （ χ^2 = 8. 609 0, P 〈 0.01 ）. Conclusions Platelet glycoprotein I bα polymorphisms are not associated with cerebral vascular thrombosis in our cohort. The Kozak genotype may be the genetic susceptibility of cerebral vascular thrombosis.

关 键 词：血小板膜糖蛋白 基因多态性 脑梗死 

分 类 号：R743[医药卫生—神经病学与精神病学]