磷脂酰肌醇3激酶在支气管哮喘大鼠气道平滑肌细胞中的表达  

Expression of PI3K in airway smooth muscle cell of asthmatic rats

作　　者：刘瑾[1] 陈俊文[1] 徐永健[1] 熊维宁[1] 白晶[1] 谢敏[1] 崔江禹[1] 郑海茹[1] 许淑云[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸内科,湖北武汉430030

出　　处：《中国病理生理杂志》2008年第4期636-639,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30500223);湖北省自然科学基金资助项目(No2007ABA157)

摘　　要：目的:探讨磷脂酰肌醇3激酶(PI3K)在支气管哮喘大鼠气道平滑肌细胞(ASMC)的表达。方法:根据随机化分配原则将16只Wistar大鼠随机均分成2组,即哮喘组和正常对照组;模型建立后分别从每只大鼠分离培养ASMC,流式细胞检测方法检测ASMC的生长分数,用免疫细胞化学荧光染色及Western blotting方法进行PI3K表达的检测,并利用图像分析系统进行半定量对比分析。结果:流式细胞仪检测发现哮喘组ASMC的S+G2/M期细胞所占细胞总数的百分比(27.90±3.44)%显著高于正常对照组(13.00±1.56)%,P<0.05;免疫细胞化学荧光染色及Western blotting方法检测发现哮喘组和正常对照组ASMC胞浆内均有PI3Kp85阳性表达,且哮喘组的表达明显高于正常对照组;大鼠ASMC中PI3K的表达与大鼠ASMC的生长分数呈显著正相关。结论:PI3K表达的增加可能在哮喘ASMC增殖中起重要作用。AIM： To investigate the expression of PI3K in airway smooth muscle cell （ASMC） of asthmatic rats. METHODS： 16 Wistar rats were divided into two groups, asthma and normal control at random. After establishment of asthmatic model, flow cytometry, immunofluorescence and Western blotting were applied to detect the growth fraction of ASMC and the expression of PI3K in cultured ASMC from each rat. RESULTS： It was revealed from flow cytometry that the ratio of S ＋ G2/M to total number of cells in asthma group [ （27.90 ±3.44） % ] was higher than that in normal control group [ （13.00 ±1.56） %, P 〈0. 051. The expression of PI3K was observed in both asthma and normal control group. However, it was much higher in asthma group than that in normal control group. There was a positive correlation between the expression of PI3K and the growth fraction in ASMC. CONCLUSION： The increased expression of PI3K might play an important role in regulating the proliferation of ASMC in asthma.

关 键 词：磷脂酰肌醇3-激酶 哮喘 气道平滑肌细胞 

分 类 号：R363[医药卫生—病理学]