小檗碱对高糖高脂诱导的NIT-1胰岛β细胞凋亡的影响  

Effects of berberine on the apoptosis of NIT-1 cells induced by high glucose and saturated fatty acids

作　　者：刘文军[1] 陆付耳[1] 董慧[1] 徐丽君[1] 王开富[1] 邹欣[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北武汉430030

出　　处：《中国病理生理杂志》2008年第4期788-791,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30472254)

摘　　要：目的:探讨小檗碱对高糖高脂诱导的NIT-1胰岛β细胞凋亡的影响。方法:采用四甲基偶氮唑蓝(MTT)比色法比较不同浓度的小檗碱对体外培养及高糖高脂诱导的NIT-1胰岛β细胞凋亡的作用,同时应用流式细胞术及原位末端核苷酸标记法(TUNEL)检测细胞凋亡。结果:不同浓度的小檗碱对NIT-1胰岛β细胞作用呈剂量依赖性:低浓度小檗碱(≤5μmol/L)对细胞无明显毒性,随浓度升高毒性作用明显。在培养基中加入高糖高脂及低浓度的小檗碱共同培养24h,小檗碱组细胞凋亡率低于高糖高脂组(P<0.01)。结论:小檗碱能抑制高糖高脂诱导的NIT-1胰岛β细胞凋亡。AIM To investigate the effects of berberine on the apeptosis of NIT - 1 cells induced by high glucose and saturated fatty acid. METHODS The influence of berberine at different concentrations on NIT - 1 cells cultured with or without high glucose and saturated fatty acid were determined and compared using MTI＂ colorimeric assay. The cell apeptotic rate was also determined by flow cytometry assay and in situ TUNEL method. RESULTS The effects of berberine at different concentrations on NIT - 1 cells showed dose - dependent, low dose （ ≤5 μmol/L） had dispensable cytotoxiciy; meanwhile, high dose showed distinct effects. On the other hand, low dose of berberine alleviated the apeptosis in NIT - 1 cells induced by high glucose and saturated fatty acid, when adding berberine to cell medium. CONCLUSION Berberine inhibited the apeptosis of NIT - 1 cells induced by high glucose and saturated fatty acid.

关 键 词：小檗碱 细胞凋亡 NIT-1细胞 

分 类 号：R363[医药卫生—病理学]