细胞固定化与硅橡胶膜渗透汽化分离耦合连续发酵制造乙醇  

Continuous Ethanol Fermentation by Cells Immobilization Associated with Pervaporation by PDMS Membrane

作　　者：丁文武[1] 伍云涛[1] 汤晓玉[1] 蒋亮[1] 邹庆[1] 杨芳[1] 徐瑶[1] 肖泽仪[1] 

机构地区：[1]四川大学化工学院,四川成都610065

出　　处：《酿酒科技》2008年第4期17-20,24,共5页Liquor-Making Science & Technology

基　　金：国家自然科学基金资助项目(20276041;20776088)

摘　　要：将硅橡胶膜(PDMS)渗透汽化分离与酵母细胞固定床耦合构成连续发酵系统,进行了乙醇发酵实验。发酵操作连续进行了378.5h,发酵液内的乙醇质量浓度最高达到了70g/L,启动PDMS膜分离后,降低并维持在50g/L左右;实验得到固定化颗粒和发酵液内的最高细胞浓度分别为1.76×1010个/g和9.8×108个/mL;乙醇体积产率3.67g/L·h,为游离连续发酵的2.1倍,葡萄糖消耗速率11.05g/L·h,为游离连续发酵的2.5倍;膜总通量400～690g/m·2h,乙醇通量为80～190g/m·2h,分离因子2.5～7.2,下游产品的平均质量浓度191.57g/L。A continuous ethanol fermentation system by immobilized yeast cells associated with prevaporation by PDMS membrane was constructed. A continuous fermentation experiment was conducted and 378.5 h continuous fermentation was achieved. The mass concentration of ethanol in the broth reached up to 70 g/L, and dropped to about 50 g/L after PDMS membrane separation started. The cell density in immobilized particles and in the broth were up to 1.76×10^10 cells/g and 9.8×10^8 cells/mL respectively. The ethanol productivity rate was 3.67 g/ （L·h） and the glucose utilization rate was 11.05 g/ （L·h）, 2.1 times and 2.5 times of continuous ethanol fermentation by free cells respectively. The total flux and the ethanol flux of the membrane were 400 g/（m^2·h）-690 g/（m^2·h） and 80 g/（m^2·h）- 190 g/（m^2·h）, while the selectivity varied between 2.5 and 7.2. The average mass concentration of ethanol of the permeate was 191.57 g/L.

关 键 词：细胞固定化 乙醇发酵 渗透汽化 膜生物反应器 

分 类 号：TQ223.122[化学工程—有机化工]