猪CACNA1S基因的多态性与胴体及肉质性状的相关分析  

Association of Polymorphisms in the Porcine CACNA1S Gene with Carcass and Meat Quality Traits

作　　者：方晓敏[1] 郭晓令[1] 徐宁迎[1] 任守文[2] 

机构地区：[1]浙江大学动物科学院,杭州310029 [2]江苏省农科院畜牧研究所,南京210004

出　　处：《畜牧兽医学报》2008年第4期405-409,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30371024);国家重大基础规划项目(2006CB102101)

摘　　要：以金华猪(40头)、皮特兰猪(30头)及其杂交产生的金皮F2代资源猪群(126头)为材料,对钙离子通道主亚基α1的编码基因(CACNA1S)第44外显子A187G突变、第37内含子A37G突变、T179C突变作PCR-RFLP检测(内切酶依次为Cfr42Ⅰ、XbaⅠ、Eco72Ⅰ),分析其对胴体及肉质性状的遗传效应。结果表明:酶切位点Eco72Ⅰ的AA基因型对眼肌面积和肉色(L)存在极显著影响(P<0.01),BB基因型对后腿肌肉重存在极显著影响(P<0.01)和大腿pH存在显著影响(0.01<P<0.05);位点XbaⅠ对背膘厚(中)存在显著影响(0.01<P<0.05),但AA、AB、BB基因型间效应差异不显著(P>0.05);位点Cfr42Ⅰ仅在金华、皮特兰纯种猪群中存在多态性,而在屠宰猪群金皮F2代则表现为BB单态型。The Jinhua pigs （40）, Pietrain pigs （30） and the second generation of crossbred of Jinhua and Pietrain （126） were used in the study, their genotype of SNPs at the position 187 of exon 44 and at the position 37 and 179 of intron 37 for CACNA1S were investigated by PCR-RFLP. The association analysis of the SNPs genotype with the meat quality traits was also conducted in the study, results showed that the genotype AA of Eco 72 Ⅰ had significant effects on M. longissimus dorsi area and meat lightness （L） （P〈0.01）, the genotype BB of Eco 72 I had significant effects on lean meat weight （P〈0.01） and pH value for ham （0.01〈P〈0.05）. The site Xba Ⅰ had significant effect on backfat thickness at the thorax and waist joint position （0.0〈P〈 0.05）, but no significant difference was found among the genotype AA, AB and BB. The site Cfr 42 Ⅰ showed polymorphisms only in Jinhua and Pietrain pigs and with the only genotype BB in the second generation of crossbred of Jinhua and Pietrain.

关 键 词：猪 CACNA1S 胴体性状 肉质性状 PCR—RFLP 

分 类 号：S828.2[农业科学—畜牧学]