缺血预处理对大鼠永久性局灶性脑缺血后HIF-1和iNOs的影响  

Effect of ischemic preconditioning on the expression of HIF-1α and iNOs after focal cerebral permanent ischemia in rats

作　　者：张会军[1] 白宏英[1] 白蓉[2] 王金兰[1] 徐辉[1] 赵源征[1] 

机构地区：[1]郑州大学二附院神经内科,郑州450014 [2]郑州大学第五附属医院康复科,郑州450052

出　　处：《中国实用神经疾病杂志》2008年第4期49-51,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的观察脑缺血预处理对低氧诱导因子-1α(hypoma inducible factor-1α,HIF-1α)和iNOs(诱导性NO合酶)在随后的永久性脑缺血中表达变化的影响,阐述脑缺血预处理对脑缺血的保护机制以及大脑在缺血后的自我保护机制。方法利用线栓法建立局灶性脑缺血-大脑中动脉闭塞模型(MCAO)。MCAO 10min作为IPC,IPC后48h制作永久性大脑中动脉梗死(PMCAO)模型。应用免疫组化法测定梗死灶周围组织中低氧诱导因子-lα和iNOs的表达水平。结果血管阻断3h后低氧诱导因子-1α和iNOs的表达水平开始升高,并且持续1周以上,缺血预处理组要比缺血组表达水平要高,2组都高于对照组,且差异有统计学意义。结论HIF-1α/iNOs系统可能作为保护因子参与脑缺血预处理对随后出现的致死性脑梗死和机体对脑缺血的保护机制。Objective To observe the expression variance of HIF-1 α and iNOs in focal cerebral permanent ischemic rats and the effect of ischemic preconditioning, and interpret the mechanisms of the protection of ischemic preconditioning before focal cerebral permanent ischemia and self-protection of the cerebrum. Methods Transient MCAO 10 min was used for IPC 48h reperfusion after IPC and before PMCAO to establish ischemic tolerance（IT）. The dynamic expression of HIF-l α and iNOs in the tissue around the necrosis was determined by immunohistochemical method. Results HIF-1 α and VEGF positive cells in the tissue around the necrosis region began to increase at 3h after vessel occlusion and last more than one week, which in IPC group were more than ischemic group, both of them were significantly more than sham operation group. Conclusion HIF-1 α/iNOs anoxic signal transduction may play a protecting role in the effect of ischemic preconditioning and the self-protection of the cerebrum after focal cerebral permanent ischemia in rats.

关 键 词：脑缺血 缺血预处理 低氧诱导因子-1 α 诱导性NO合酶 

分 类 号：R-332[医药卫生]