牛磺酸抑制糖尿病大鼠视网膜Muller细胞VEGF表达的研究  

Study on Taurine in Inhibition of Expression of VEGF in Retinal Muller Cells of Diabetic Rats

作　　者：曾凯宏[1] 许红霞[1] 糜漫天[1] 张亚洁[1] 陈芳[1] 陈卡[1] 

机构地区：[1]第三军医大学营养与食品卫生学教研室营养与食品安全重庆市市级重点实验室,重庆400038

出　　处：《现代生物医学进展》2008年第4期601-604,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(No.30571570)

摘　　要：目的:进一步了解糖尿病引起视网膜受损的分子机制、探讨牛磺酸保护糖尿病大鼠视网膜损伤的可能机制。方法用链脲佐菌素诱导SD大鼠惠糖尿病,分为正常对照组、糖尿病组、1%牛磺酸干预糖尿病组、5%牛磺酸干预糖尿病组、胰岛素治疗糖尿病组。正常对照组、糖尿病组、胰岛素治疗组饲以基础饲料,牛磺酸干预组饲以基础饲料分别添加1%、5%牛磺酸的饲料喂养,胰岛素治疗组每天皮下注射20U/kg胰岛素。在第2周、1月、2月、3月取视网膜,用RT-PCR、免疫组织荧光化学、Western-blotting检测视网膜Muller细胞VEGF mRNA和蛋白表达情况。结果:经链脲佐茵素诱导患糖尿病2周后,SD大鼠视网膜Muller细胞VEGF mRNA和蛋白表达增加,且随病程的延长表达量有持续增加趋势(P<0.05)。患糖尿病3月后,整个视网膜中VEGF免疫染色明显增强,尤以外网状层(OPL)、内网状层(IPL)和视网膜外段变化最明显。牛磺酸干预糖尿病1月后。大鼠视网膜Muller细胞VEGF mRNA和蛋白的表达下调(P<0.05)。结论:牛磺酸抑制糖尿病患者视网膜Muller细胞VEGF的表达,减轻糖尿病引起的视网膜损害。Objective： To investigate the effects of taurine intervention on retinal vascular endothelial growth factor （VEGF） expression in retinal Muller cells of diabetic rats and the mechanism of taurine in the protection of diabetic rats from retinopathy. Methods： The rats were given intraperitoneal injection of streptozotocin and then divided into 5 groups, i.e. normal control group （NC）, diabetes mellitus group （DM）, 1% taurine intervention diabetes mellitus group （DM＋1%T）, 5% taurine intervention diabetes mellitus group （DM＋5%T）, insulin treatment diabetes mellitus group （DM＋I）. 2 weeks, 1 month, 2 months and 3 months after DM induction, VEGF expression in the detected retinal Muller cells were determined by RT-PCR, immunohistofluorescence and western-blotting. Results： From 2 weeks to 3 months, the mRNA and protein expression of VEGF gradually increased in diabetic retinal Muller cells. The VEGF expression in outer plexiform layer （OPL）, inner plexiform layer （IPL）and outer segment of retina increased at 3 months after DM. A month after Taurine intervention, the mRNA and protein expression of VEGF in retinal Muller cells of diabetic rats down-regulated （P〈0.05）. Conclusions： Taurine can down-regulate the mRNA and protein expression of VEGF in retinal Muller cells of diabetic rats, and can relieve diabetic rats from retinopathy.

关 键 词：糖尿病 视网膜 牛磺酸 血管内皮生长因子 

分 类 号：R587.1[医药卫生—内分泌]