大鼠源性AT1受体自身抗体的制备  

Autoantibody against the Angiotensin Ⅱ Type 1 Receptor Can be Induced by Immunization with Rat AT 1 Receptor Peptide

作　　者：卢彩宝[1] 赵洪雯[1] 刘宏[1] 李敛[1] 吴雄飞[1] 

机构地区：[1]第三军医大学西南医院肾科,重庆400038

出　　处：《现代生物医学进展》2008年第4期626-628,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨大鼠源性的血管紧张素Ⅱ1型受体(AngiotensinⅡtype 1 receptor,AT1受体)多肽免疫大鼠能否产生AT1受体自身抗体(autoantibodies against the angiotensinⅡtype 1 receptor,AT1-AA),AT1-AA对自身组织的损伤作用。方法:22只雄性SD大鼠随机分为二组,免疫组16只,对照组6只。AT1受体细胞外第二环合成肽与载体血蓝蛋白偶联后再混合佐剂制备免疫液免疫大鼠,每隔3周加强免疫一次;对照组实行"假性免疫",但"免疫液"中不含有AT1受体细胞外第二环合成多肽。ELISA法检测血清AT1受体自身抗体;观察肾脏、心脏、肝脏和腹主动脉组织光镜和肾脏组织电镜的病理变化;生物机能实验鉴定AT1受体自身抗体;试验期15周。结果:免疫组大鼠于第3次加强免疫后出现AT1受体自身抗体,第4次加强免疫后抗体滴度进一步升高;免疫组大鼠血清可以增加新生鼠心肌细胞跳动频率;光镜及电镜未观察到明显的心脏、肾脏和肝脏病理改变。结论:大鼠源性AT1受体多肽能够诱导大鼠产生AT1受体自身抗体,此抗体对自身组织的损伤作用不明显。Objective： To investigate whether specific anti-AT1 receptor （Angiotensin Ⅱ type 1 receptor, AT1 receptor） antibodies can be induced by immunization with rat AT1 receptor peptide and to examine their damaging effects on host tissues. Method： Twenty two male rats were used and randomly divided into two groups： a control group （n=6） and an AT1 receptor peptide immunized group （n =16）. For rats in immunized group, the second extracellular loop of AT1 receptor peptide was coupled to keyhole limpet hemocyanin for immunization with Freund＇s adjuvant. Booster injections were given every four weeks. For rats in control group, solution of the same amount except the second extracellular loop of AT1 receptor peptide were given at the same time points and duration as in immunized group. Antibody against the second extracellular loop of AT1 receptor peptide was examined by ELISA. Pathological changes in heart, kidney, liver and abdominalis aorta were examined under light microscope and morphological changes in ultrastructural pathology of kidney were detected under electron microscope. Biofunctional studies were performed to identify the auto-antibodies of AT1 receptor. The observational study last for fifteen weeks. Results： Auto-antibodies against AT1 receptor were detected after three doses of booster injection, which was further increased by the fourth immunization. Anti-serum from immunized rats can boost the beating frequency of cardiac cells of neonatal rats. No significant pathological changes were observed in the chosen tissues. Conclusion： AT1 receptor peptide from rats can induce auto-antibodies by immunization procedure, which has no apparent damaging effects on host tissues.

关 键 词：AT1受体 自身抗体 多肽 免疫 

分 类 号：R392.4[医药卫生—免疫学] R593[医药卫生—基础医学]