大鼠脑基质金属蛋白酶-9表达与癫痫发病机制关系的研究  

A Study on the Relationship between the Expression of Matrix Metalloproteinase-9 in Rat Brain and the Pathogenesis of Epilepsy

作　　者：郑乃智[1] 张慧娟[2] 王敏[1] 

机构地区：[1]山东省青岛大学医学院附属青岛市市立医院神经科,266011 [2]山东省烟台市烟台山医院神经科

出　　处：《医学研究杂志》2008年第4期70-73,共4页Journal of Medical Research

摘　　要：目的研究大鼠脑内基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的表达与癫痫免疫学发病机制的关系。方法用免疫组化方法观察正常和癫痫发作不同时间后大鼠海马区MMP-9免疫反应阳性表达。同时观察致痫前给予地塞米松对海马区MMP-9免疫反应阳性表达的影响。结果正常情况下,大鼠海马区MMP-9免疫反应阳性表达较少。致痫后24h大鼠海马区MMP-9免疫反应阳性表达增强。致痫后72h齿状回(dentate gyrus,DG)MMP-9免疫反应阳性表达较致痫后24h增强(P<0.01);而安蒙角(corner ammon,CA)区,该表达无显著变化(P>0.05)。地塞米松对海马区MMP-9免疫反应阳性表达有抑制作用。结论脑内MMP-9的表达通过影响免疫反应,引起海马区神经元反应性改变和突触重塑,调节神经细胞兴奋性,参与癫痫免疫学发病机制。地塞米松对这一过程有显著的抑制作用。Objective To study the relationship between the expression of matrix metalloproteinase -9 in rat brain and pathogenesis of epilepsy. Methods The expression of matrix metalloproteinase - 9 in hippocampus was observed by immunochemlstry in normal and epilepsy rat. Meanwile, the effects of dexamethasone before epilepsy on the expression of MMP -9 in hippocampus was evaluated. Results The expression of MMP - 9 in hippocampus were fewer in normal rat brain. The expression of MMP - 9 had increased in hippocampus after epilepsy 24h. It had increased obviously in dentate gyrus （DG） after epilepsy 72h than 24h（ P 〈0.01 ） , while there was no obvious change about the expression of MMP- 9 in Comer Ammon （CA）（P 〉 0.05）. Dexamethasone suppressed the expression of MMP- 9 in hippocampus. Conclusion The expression of MMP - 9 in rat brain participates in immunopathogenesis of epilepsy by affecting brain immunoreaction,leading to the change of neuronal response and synapse remodeling in hippocampus, regulating brain nervous cell excitability. Dexamethasone plays a prominent suppressant role in the processes.

关 键 词：基质金属蛋白酶-9 地塞米松 癫痫 发病机制 

分 类 号：R735.2[医药卫生—肿瘤] R692[医药卫生—临床医学]