雌激素α和β受体在大鼠结膜上皮和Henle腺上的分布  

DISTRIBUTIONS OF ESTROGEN RECEPTORS ALPHA AND BETA IN THE CONJUNCTIVA EPITHELIUM AND HENLE GLAND OF THE RATS

作　　者：李联祥[1] 李沛[2] 王秋丽[1] 

机构地区：[1]河北工程大学医学院人体解剖学与组织胚胎学教研室 [2]河北工程大学附属医院,邯郸056002

出　　处：《解剖学报》2008年第2期235-237,共3页Acta Anatomica Sinica

基　　金：河北省教育厅自然科学基金资助项目(2002160)

摘　　要：目的阐明雌激素α、β受体在大鼠结膜上皮和Henle腺的分布。方法选择22例青春期SD雌性大鼠上眼睑标本,采用免疫组织化学方法对结膜上皮和Henle腺雌激素α、β受体进行检测;同时采用放射免疫分析技术检测大鼠血清雌二醇的浓度。结果22例大鼠中,19例大鼠的结膜上皮雌激素α受体呈阳性表达,17例大鼠的结膜上皮雌激素β受体呈阳性表达。18例大鼠Henle腺深部的基底细胞雌激素α受体呈阳性表达,17例大鼠的Henle腺深部的基底细胞雌激素β受体呈阳性表达。在所有的大鼠中,Henle腺的杯状细胞雌激素α、β受体均呈阴性表达。雌激素α、β受体在结膜上皮阳性表达率间比较,差异无显著性(P>0.05);雌激素α、β受体在Henle腺深部的基底细胞阳性表达率间比较,差异亦无显著性(P>0.05)。正常大鼠血清雌二醇水平经检测含量为(22.13±3.54)ng/L。结论雌激素及其受体对大鼠结膜上皮、Henle腺深部的基底细胞具有重要的调控作用,但对Henle腺的杯状细胞分泌黏液功能无直接调节作用。Objective To clarify the distribution of estrogen receptor alpha（ERα） and estrogen receptor beta（ERβ） in conjunctiva epithelium and Henle gland of rats. Methods Among the twenty-two eyelid specimens of puberty female SD rat were studied by immunohistochemical method.The serum estradiol level of rats was examed by radioimmunoassay. Results Among the twenty-two eyelid specimens,nineteen were positive for estrogen receptor alpha and seventeen for estrogen receptor beta in conjunctiva epithelium.Eighteen were positive for estrogen receptor alpha and seventeen for estrogen receptor beta basal cell in the deep part of Henle gland.All were negative for the two receptors in the goblet cell of Henle gland.The positive rates for the two receptores in conjunctiva epithelium do not statistically show a significant difference（P〉0.05）;the positive rates for the two receptors in the basal cell in the deep part of the Henle gland do not statistically show a significant difference either（P〉0.05）.The serum estradiol level of rat was（22.13±3.54）ng/L.Conclusion Those results showed that estrogen and its receptors has an important role in adjusting conjunctiva epithelium and the basal cell in the deep part of Henle gland,while they do not directly adjust the secretion function of the goblet cell of Henle gland.

关 键 词：结膜 Henle腺 雌激素受体 黏蛋白 免疫组织化学 大鼠 

分 类 号：R777.1[医药卫生—眼科] R335[医药卫生—临床医学]