蛤蚧皮质加厚区的形态解剖及三维重建  

MORPHOLOGICAL ANATOMY AND THREE-DIMENSIONALRECONST RUCTION OF THE PALLIAL THICKENING IN THE LIZARD GEKKO GECKO

作　　者：伍冠一[1] 廖锦锋[2] 陈静[3] 潘盛武[1] 马殷华[4] 唐宗湘[1] 

机构地区：[1]广西师范大学生命科学学院,桂林541004 [2]广西桂林医学院生物技术学院,桂林541004 [3]北京大学南宁附属实验学校,南宁530022 [4]广西师范大学学报编辑部,桂林541004

出　　处：《解剖学报》2008年第2期243-247,共5页Acta Anatomica Sinica

基　　金：广西留学回国人员基金(0448012)资助

摘　　要：目的明确蛤蚧皮质加厚区的位置、核团的形态及与比邻结构的关系,为研究皮质加厚区的功能提供依据。方法对蛤蚧脑行冷冻冠状连续切片(厚60μm),Nissl染色,对每张含有皮质加厚区的切片进行摄片和测量,选取其中1套用于三维重建,重组软件为3D MAX。结果1.皮质加厚区位于端脑吻侧端,前背侧室嵴外侧,外侧皮层内侧和背侧皮层腹侧。皮质加厚区的吻尾长(912.67±110.96)μm(n=10),体积(0.1430±0.0414)μm3(n=10)。2.皮质加厚区从吻侧端到尾侧端大约可分为前段、中段、后段和末段。后段的背腹径最长,可分为背侧部和腹侧部,两部分的细胞分界清晰。结论皮质加厚区为一吻尾径长于内外径的狭长片状结构,背侧缘较腹侧缘平滑;加厚区的尾侧端比吻侧端大,且尾端的后段分离成背侧和腹侧两个细胞群。Objective To clarify the position,figure and connections with adjacencies in the pallial thickening（Pth）,and provide essential parameters for its function study. Methods The coronal serial sections of 60μm thickness in gekko gecko brain were made by cryo-microtome,and Nissl staining was used.Pictures were taken in each coronal section containing the Pth and the size of Pth in each section was measured.One of them was chosen for the three-dimensional reconstruction.3D MAX was used as the tool software to rebuild the nucleus. Results 1.The Pth was located in the rostral part of the telencephalon,the lateral part of anterior dorsal ventricular ridge,the medial part of the lateral cortex and the ventral part of the dorsal cortex.The length of Pth from the rostral to the caudal end was（912.67±110.96）μm（n=10）,the cubage of Pth was about（0.1430±0.0414）μm^3（n=10）.2.The Pth could be divided into four segments,the anterior,the middle,the posterior and the terminal segments from the rostral to the caudal end.In the posterior segment,its dorsoventral axis was the longest,and could be divided into two parts： the dorsal and the ventral parts.The boundary of the two parts was clear.Conclusion The Pth is a long,narrow and flat structure;its rostrocaudal axis is longer than its dorsoventral axis,and its dorsal edge is smoother than its ventral edge.In the Pth,its caudal region is larger than its rostral region,and the posterior segment in the caudal region is divided into the dorsal and the ventral cell populations.

关 键 词：皮质加厚区 三维重建 蛤蚧 

分 类 号：Q954.52[生物学—动物学]