蛇毒神经生长因子对大鼠视网膜缺血再灌注损伤的神经保护研究  

An experimental study of protection effect of viper venom nerve growth factor on experimental retinal ischemia/reperfusion injury

作　　者：王瑛[1] 刘金华[1] 唐秀武[2] 

机构地区：[1]广西医科大学第一附属医院眼科中心,广西壮族自治区南宁市530021 [2]广西壮族自治区民族医院眼利

出　　处：《中国实用眼科杂志》2008年第2期164-167,共4页Chinese Journal of Practical Ophthalmology

基　　金：本课题受国家自然科学基金资助（资助号：30460138）

摘　　要：目的探讨玻璃体腔内注射蛇毒神经生长因子，对实验洼视网膜缺血再灌注损伤是否具有神经保护作用。方法采用升高大鼠眼内压的方法，制作实验性视网膜缺血再灌注损伤模型。实验组和对照组分别注入蛇毒神经生长因子和平衡盐溶液，应用图像分析系统计数视网膜神经节细胞和测量视网膜内层厚度，透射电镜观察视网膜超微结构。结果视网膜缺血再灌注开始，实验组大鼠视网膜水肿、视网膜内层厚度变薄和视网膜神经节细胞（RGCs）数目减少较对照组轻，并且实验组于再灌注24h后RGCs数目逐渐增多，48h后视网膜内层厚度逐渐增加。再灌注后168h，对照组大鼠视网膜内层厚度及RGCs数目明显低于实验组，差异显著有统计学意义（P＜0．05）；电镜观察对照组再灌注后24h出现膜盘排列紊乱、变形，神经纤维层内大量的线粒体肿胀、空泡化和RGCs核染色质浓缩、边集，出现凋亡小体，胞浆内细胞器空泡化且大量减少。而实验组膜盘排列尚整齐，RGCs轻度肿胀，胞浆内细胞器较丰富，神经纤维层中的线粒体轻度肿胀，微管结构较清楚。结论通过向大鼠玻璃体腔内注射蛇毒神经生长因子可以减轻视网膜内层的损伤，对实验性视网膜缺血再灌注损伤有神经保护作用。Objective To evaluate the protection effect of viper venom nerve growth factor（vNGF） injected into the vitreous cavity on experimental retinal ischemia/reperfusion injury （RIR）. Method The Spregue- Dawley（SD） rat model of experimental retinal ischemia/reperfusion injury was made by increasing the intraocular pressure. The rats were divided into normal, treatment and control groups randomly. At the beginning of reperfusion, 20μl （100BU） of vNGF was injected into the experimental group and 20μl of BSS was injected into the vitreous cavity in Control group. The histological and ultrastructural changes in retina at different time after reperfusion were observed. The retinal ganglion cell number was counted and the thickness of inner layer of retina was measured by using image diagnosis system. The ultrastructural morphology was examined under transmission electron microscope（TEM）. Result In the period of retinal ischemia/reperfusion injury and the edematous status of retina and the thickness of retinal inner layer declined and the RGC numbers decreased of experimental group was lighter than that of the Control group. In addition, The RGC numbers increased gradually since 24 hours after reperfusion and the thickness of retinal inner layer increased gradually since 48 hours after reperfusion in experimental group. At 168 hours after reperfusion, the thickness of the retinal inner layer and the RGC numbers of Control group were obviously lower than that of experimental group, the differences of statistical were very importance（P〈0.01）. At 24 hours after reperfusion of Experimental group, the arrangement of the membrane disc became disordered and distorted. Most chondriosomes of nerve fiber layer were swelling and vacuolating, the chromatin of RGCs was concentrated and marginate, theapoptotic body can be found, organelles in the cytoplasm were swelling, vacuolating and decreasing. While in the experimental group,the arrangement of the membrane disc were tidy, the swell of RGCs was more slight,

关 键 词：缺血再灌注损伤 蛇毒神经生长因子 视网膜 

分 类 号：R774.1[医药卫生—眼科] R651.3[医药卫生—临床医学]