肺应秋生理机制的免疫学实验研究  

Immunological experimental study on physiological mechanism of "lung corresponding to autumn"

作　　者：马淑然[1] 吴同玉[1] 刘燕池[1] 郭霞珍[1] 李晓君[1] 刘晓燕[1] 梁怡[1] 赵树宏[1] 苏薇[1] 徐巍华[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2008年第3期167-170,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局资助项目(No04-05JL01);教育部科学技术重大研究项目(No105030);国家自然科学基金资助项目(No30672567)

摘　　要：目的探讨中医"肺应秋"生理机制的免疫学内涵,为藏象本质研究提供新思路,为呼吸系统季节性发病的病理生理机制的认识提供实验依据。方法采用松果腺摘除大鼠模型,测定春分秋分大鼠脾脏指数和胸腺指数(用称重法)、脾脏T淋巴细胞转化率(用噻唑蓝比色法)、肺泡巨噬细胞吞噬功能(用鸡红细胞吞噬法)、肺泡灌洗液的γ-干扰素(IFN-γ,用ELISA法)、肺组织白细胞介素-10(IL-10,用放射免疫法)。结果①生理组脾脏指数和胸腺指数,秋分明显低于春分,有非常显著差异;伪手术组秋分低于春分,有显著差异;手术组春分和秋分时差异不明显。②生理组、伪手术组、手术组大鼠脾脏T淋巴细胞转化率在秋分时均明显低于春分,有非常显著差异(P<0.01)。无论春分或秋分,伪手术组和手术组大鼠脾脏T淋巴细胞转化率均明显高于当季的生理组(P<0.01),差异具有非常显著性。③生理组肺泡巨噬细胞吞噬功能秋分明显低于春分,有非常显著差异;伪手术组与手术组春分和秋分则无差异。④生理组肺泡灌洗液的IFN-γ秋分略低于春分,但无明显差异;伪手术组和手术组秋分略高于春分,但差异不明显。⑤生理组肺组织IL-10秋分略低于春分,但无明显差异;伪手术组秋分略高于春分,但差异不明显。手术组秋分略低于春分,但差异也不明显。结论①机体免疫功能及肺局部免疫功能存在秋低春高的季节节律,这可能是秋季季节性呼吸系统多发病的病理生理学基础之一。②松果腺在机体免疫功能季节性节律变化中具有一定的调节作用。③肺应秋的本质是机体整体及肺的局部免疫功能在秋季调节能力降低,松果腺-褪黑素是肺免疫功能季节性节律变化的中介物质之一。objective To discuss the immunological connotation of modulating mechanism of ＂ lung corresponding to autumn＂, and to provide new ideas for the essence of Zangxiang and experimental basis （using MTT assay） , alveolar macrophages phagocytosis （using phagocytosis of chicken erythrocytes） , IFN-γ in BALF（using ELISA assay）, IL-IO in the lung tissues（using RIA）. Results （1） The indexes of spleenand thymus in the normal group and the sham operated group were lower on autumn equinox than on vernal equinox with significant differences. The indexes of spleen and thymus in the model group showed no significant differences between autumn equinox and vernal equinox. （2） T lymphocyte transformation rate of spleen in all three of the groups were lower on autumn equinox than on vernal equinox with the remarkable differences （P 〈0. 01 ）. T lymphocyte transformation rates of spleen sham operated group season （P 〈0.01）. and the model group were obviously higher than in the normal group in the （3） The alveolar macrophages phagocytosis in the normal group was obviously on autumn equinox than on vernal equinox with the significant differences. The differences on equinox and autumn equinox were disappeared in the sham operated group and the model levels in BALF in the normal group were slightly lower on autumn equinox than on vernal in the salne lower vernal group. （4） IFN-γ equinox without the significant differences. They were slightly higher on autumnal equinox than on ,vernal equinox in the sham operated group and the model group without the significant differences. （5） IL-10 levels of lung tissues in the normal group were slightly lower on autumn equinox than on vernal equinox without the significant differences. They were slightly higher on autumnal equinox than on vernal equinox in the sham operated group without the significant differences. They were slightly lower on autumn equinox than on vernal equinox in the model group without the significant differences. Conclusion

关 键 词：肺应秋 松果腺 免疫功能 大鼠 

分 类 号：R221.3[医药卫生—中医基础理论]