大豆异黄酮苷元检测方法的研究  

Instant determination of soy isoflavone aglycones

作　　者：张磊[1] 金桂芳[1] 杨红[1] 习志刚[1] 

机构地区：[1]广东药学院基础学院,广东广州510006

出　　处：《广东药学院学报》2008年第1期30-32,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省社会发展计划工业攻关项目(2005B10401005);中山市科技计划项目(2006A120)

摘　　要：目的建立大豆异黄酮苷元含量测定方法。方法采用HPLC法,Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相:甲醇-0.3%磷酸溶液(体积比48∶52),流速0.7 mL/min,测定波长260 nm,柱温25℃。结果制备的大豆异黄酮苷元总含量为70.98%;大豆苷元、黄豆黄素和染料木素平均回收率分别为98.7%、98.9%和99.2%,RSD分别为2.1%、1.6%和1.4%。结论所建立的测定方法重现性好、可靠,可用于3种大豆异黄酮苷元的含量测定。Objective To establish an HPLC method for the content determination of isoflavone aglycones. Methods Isoflavone aglycones were analyzed on a Diamonsil C18 column （250 mm×4.6 mm,5 μm）. A mixture of MeOH-0. 3% H3PO4 （48： 52） was used as the mobile phase with a flow rate of 0.7 mL/min. The detecting wavelength was 260 nm at 25℃. Results The content of isoflavone aglycones in the sample was 70.98%. The average recoveries of genistein,daidzein and glycitein were 99.2% ,98.7% and 98.9% with RSD 1.4% ,2.1% and 1.6% ,respectively. Conclusions This method can be used for the quality control of soy isoflavone aglycones.

关 键 词：大豆异黄酮苷元 高效液相色谱法 含量测定 

分 类 号：R927[医药卫生—药学]