Analysis on promoter elements of cpc operon from Arthrospira platensis  

作　　者：LU Yongzhong ZHANG Xuecheng 

机构地区：[1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China [2]Qingdao University of Science and Technology, Qingdao 266042, China

出　　处：《Acta Oceanologica Sinica》2008年第1期85-91,共7页海洋学报（英文版）

基　　金：The National Natural Science Foundation of China under contract No.30471317.

摘　　要：On the basis of bioinformatics analysis, six putative promoters were isolated from the upstream sequence of c-phycocyanin gene of Arthrospira platensis FACHB341 through site-directed mutagenesis, and the transcriptional pattern of c-phycocyanin gene was determined by SmartRace. Results showed that each isolated promoter drived the expression of gfp in E. coli, and Promoter Ⅲ was the strongest one according to the GFP level. Only one transcript of c-phycocyanin gene was found under experimental conditions, and the transcription start site G was located at the - 285 bp upstream of the start codon, from which it could be inferred that the transcript was from the promotion of Promoter Ⅰ.On the basis of bioinformatics analysis, six putative promoters were isolated from the upstream sequence of c-phycocyanin gene of Arthrospira platensis FACHB341 through site-directed mutagenesis, and the transcriptional pattern of c-phycocyanin gene was determined by SmartRace. Results showed that each isolated promoter drived the expression of gfp in E. coli, and Promoter Ⅲ was the strongest one according to the GFP level. Only one transcript of c-phycocyanin gene was found under experimental conditions, and the transcription start site G was located at the - 285 bp upstream of the start codon, from which it could be inferred that the transcript was from the promotion of Promoter Ⅰ.

关 键 词：C-PHYCOCYANIN SmartRace TRANSCRIPTION PROMOTER CYANOBACTERIUM 

分 类 号：Q949.2[生物学—植物学]