家兔非酒精性脂肪肝肝纤维化形成中层粘连蛋白及透明质酸的变化  

Successive observation of laminin and hyaluronic acid on the formation of non-alcoholic fatty liver fibrosis in rabbits

作　　者：赵迎庆[1] 王炳元[2] 

机构地区：[1]沈阳市第五人民医院消化内科,辽宁省沈阳市110023 [2]中国医科大学第一附属医院消化内科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2008年第8期823-828,共6页World Chinese Journal of Digestology

摘　　要：目的:研究非酒精性脂肪肝家兔肝纤维化形成过程中肝窦壁层粘连蛋白(LN)及透明质酸(HA)的变化.方法:用高脂饲料诱发脂肪肝的方法建立家兔非酒精性肝病动物模型,取肝组织用扫描电镜、特殊染色等进行病理组织形态学观察:免疫组化检测LN;放射免疫法测定血清HA,研究非酒精性脂肪肝家兔在肝纤维化形成过程中肝窦壁基底膜成分的动态变化.结果:肝窦毛细血管化,即窗孔消失、基底膜形成;肝窦壁LN表达(面积百分比)和血清HA含量(mg/L)均随着肝纤维化形成逐渐增加,在8 wk末阳性最强,均与对照组有显著性差异(LN:25.2±1.0 vs 5.1±0.7,P<0.01;血清HA:1422.18±20.9 vs 1189.3±13.1,P<0.01),停止造模后逐渐下降,但停药8 wk均强于正常组.结论:家兔肝纤维化形成过程中,肝窦壁功能性基底膜被破坏,发生肝窦毛细血管化.血清HA含量的变化与电镜下SEC去窗孔的改变,以及LN的变化相一致.AIM： To observe the changes of laminin （LN） and hyaluronic acid （HA） on the formation of non-alcoholic fatty liver fibrosis in rabbits. METHODS： Firstly, the rabbit models of fatty liver and liver fibrosis were set up by hypercholesteremia. Liver tissues were collected and the histopathological changes were observed using transmission endoscopy and staining methods. Immunohistochemistry was used to detect LN expression and radioimmunoassy was used the measure serum HA concentration. Successive observation was used to outline the dynamic changes of basement membrane of hepatic sinusoid during the formation of non-alcoholic fatty liver fibrosis in rabbits. RESULTS： Sinusoidal capillarization was characterized by defenestration of sinusoidal endothelial cells （SEC） and formation of basement membrane. Along with the formation of liver fibrosis, the positive expression （area percentage） of LN on sinusoidal wall and the concentration （mg/L） of serum HA increased gradually, and both of them reached the peak at 8 wk; there were significant differences in contrast with those in the normal group （LN： 25.2 ± 1.0 vs 5.1 ± 0.7, P 〈 0.01; serum HA： 1422.18 ± 20.9 vs 1189.3 ± 13.1, P 〈 0.01）. After stopping high-sterone feeding, the positive expression of LN and concentration of HA decreased gradually. CONCLUSION： The changes of serum HA concentration are in accordance with the defenestration of hepatic sinusoid and LN changes. LN and HA may be used as cardinal markers for hepatic sinusoidal capiUarization and liver fibrosis.

关 键 词：层粘连蛋白 透明质酸 非酒精性脂肪肝 肝纤维化 肝窦毛细血管化 

分 类 号：R575.5[医药卫生—消化系统] R575.2[医药卫生—内科学]