细小病毒H-1对人肝癌移植瘤的抑制及其组织学与组织化学的研究  

INHIBITION OF PARVOVIRUS H-1 ON TRANSPLANTABLE HUMAN HEPATOMA AND ITS HISTOLOGICAL AND HISTOBIOCHEMICAL STUDIES

作　　者：施志仪[1] 马承武 黄建[1] 林万敏[1] 董荣春[2] 罗祖玉[1] 

机构地区：[1]复旦大学生命科学学院,上海200433 [2]第二军医大学病理解剖教研室,上海200433

出　　处：《实验生物学报》1997年第3期247-259,共13页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金~~

摘　　要：本文用人肝癌裸小鼠ＱＧＹ－９２０４移植模型为材料，进行了细小病毒Ｈ－１抑瘤的组织学、组织化学及分子生物学研究所。A transplantable human hepatoma model, the QGY-9204. was used in this study. The growth kinetics of hepatoma in nude mice were compared after injection of parvovirus H-1 into the tumor growth. Significant difference in growth curves were seen between injected groups with H-1 dosages of 5×107 PFU and 5×108 PFU and that of control. It indicated that parvovirus H-1 was capable of suppressing the growth of human hepatoma. Previous studies showed H-1 is on-cotropic, oncosuppressive and oncolytic. For histological, ultrastructural and his-tochemical examinations, transplantable hepatomas were taken at different time interval post H-1 (1×108 PFU per tumor growth) injection. For H-l DNA amplification and H-1 nonstructural protein expression, PCR and ABC approach in hepatoma paraffin sections were used. The H-1 treated groups exhibited obvious signs of necrosis. It started on 3 rd day post infection (3 d.p.i.) and the area of necrosis enlarged consecutively on 7 d.p.i., 10d.p.i. and 14 d. p. i., but none was seen in saline-injected group even on 14 d.p.i. H-1 virions were also detected in the damaged tumor cells with numerous vacuoles in cytoplasm. Specific band (908 bp) of H-1 DNA and ABC im-munostaining indicated H-1 DNA replication and NS-1 expression in tumors of treated groups, their time course was well in accordance with that process of necrosis. These results suggest that parvovirus H-1 promotes tumor necrosis by its DNA replication and cytotoxic NS-1 protein expression, and thus, it inhibits hepatoma growth and induces oncosuppression and oncolysis.

关 键 词：肝癌 移植瘤 细小病毒H-1 肿瘤抑制 

分 类 号：R735.705[医药卫生—肿瘤] Q939.408[医药卫生—临床医学]