中度嗜盐菌的分离鉴定及对高盐制革废水处理的强化  

Isolation and Identification of Moderately Halophilic Bacterium and Enhanced Treatment of Hypersaline Tanning Wastewater by the Bacterium

作　　者：李维国[1] 马放[1] 魏利[1] 张大伟[2] 柳锋[2] 王光玉[2] 苏俊峰[1] 

机构地区：[1]哈尔滨工业大学城市水资源与环境国家重点实验室,黑龙江哈尔滨150009 [2]哈尔滨工业大学海洋学院,山东威海264209

出　　处：《华南理工大学学报（自然科学版）》2008年第3期89-94,共6页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家973项目(2004CB185050);黑龙江省重大科技攻关项目(CC05S301)

摘　　要：为探索中度嗜盐菌在高盐有机制革工业废水处理中的应用,解决高盐有机制革废水处理这一难题,从某晒盐池盐水中分离获得一株嗜盐菌株YS-1,对该菌株进行原子力显微镜观察、生理生化测定、全细胞脂肪酸分析和16S rDNA序列同源性分析.研究表明,YS-1菌株16S rDNA序列与Halomonas sp.(AB167061)的亲缘关系最为接近,结合上述其它各项测定结果,确定该菌株为盐单胞菌属(Halomonas sp.),属中度嗜盐菌.在SBR反应器中对其进行强化高盐制革废水处理试验,同时结合生物活性碳技术,结果表明,含盐9.3%、CODCr为1738mg/L的高盐制革废水,经168 h的处理后CODCr去除率为86%,经216 h处理后CODCr去除率为98%,表明该菌株具有强化高盐制革废水处理的功能.将分离筛选的嗜盐微生物与生物活性碳技术相结合,可构建"嗜盐生物活性碳"技术,来强化高盐制革废水的处理.In order to investigate the application of moderately halophilic bacterium to the treatment of hypersaline organic tanning wastewater, a halophilic bacterium YS-1 was isolated from the salt water of a salina. The atomic force microscopy （AFM） observation, the physiologic-biochemical index measurement, the whole-cell fatty acid analysis and the 16S rDNA sequence homology identification for the bacterium were then carried out. The results show that the closest relative of YS-1 strain is Halomonas sp. （AB167061）, meaning that YS-1 strain is a novel moderately halophilic species of Halomonas sp.. Moreover, according to the results of hypersaline tanning wastewater treatment by the identified bacterium combined with the biological activated carbon （BAC） technology in a sequencing batch reactor （SBR）, it is found that, for the wastewater with an initial salt content of 9.3% and an initial CODer of 1378mg,/L, the CODcr removal reaches 86% after the treatment for 168h, and rises to 98% after 216h. It is thus concluded that YS-1 strain can enhance the treatment of hypersaline tanning wastewater, and that a ＂halophilic-BAC technology＂ combining the screened bacterium and the BAC technology is feasible in the treatment of hypersaline tanning wastewater.

关 键 词：中度嗜盐菌 分离 鉴定 高盐制革废水 废水处理 

分 类 号：X172[环境科学与工程—环境科学]