宫颈癌P53蛋白及DNA含量的流式细胞分析  

Flow Cytometry Analysis of P53 Protein and DNA Content in Cervical Cancer

作　　者：李魁秀[1] 房朝晖[1] 吕俊玲 王争国[1] 左连富[2] 

机构地区：[1]河北医科大学附属四院,050011 [2]河北省肿瘤研究所

出　　处：《中华物理医学杂志》1997年第3期168-170,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的:探讨P53蛋白表达及DNA含量与宫颈癌临床病理及预后的关系。方法:用流式细胞术分析60例宫颈癌和10例正常宫颈标本的P53蛋白表达及DNA含量。结果:①宫颈癌细胞的P53蛋白表达及DNA含量明显高于正常宫颈上皮细胞。②P53蛋白过度表达及异倍体与临床分期及肿瘤大小显著相关。③P53蛋白的荧光强度≥1.5及DNA含量≥1.3者预后差。结论:用流式细胞术测宫颈癌患者的P53蛋白表达及DNA含量快速、准确,对宫颈癌早诊、协助治疗和判断预后有重要意义。Objective: To study expression of P53 protein and DNA content and their relation to clinicopathology and prognosis of cervical cancer. Methods: Expression of P53 protein and DNA content of 60 primary cervical carcinomas and 10 normal cervical specimens were analysed by flow cytometry. Results: ① Fluorescence index(FI) of P53 protein and DNA index(DI) in cervical cancer were significantly higher than that in normal cervical epthelium (P<0.05). ② Overexpression of P53 protein and aneuploid in cervical cancer had significant correlation with tumor stage and tumor volume. ③ FI protein of P53≥1.5 or DI≥1.3 showed bad prognosis. Conclusion: Flow eytometry can quickly and accurately detect P53 protein and DNA content of the patients with cervical cancer, providing an important evaluation value for diagnosis、treatment and prognosis of cervical carcinoma.

关 键 词：子宫颈肿瘤 P53蛋白 DNA 流动血细胞计数 

分 类 号：R737.330.2[医药卫生—肿瘤]