免疫层析法快速测定鸡肉和鸡蛋中磺胺类药物残留  

Rapid detection of sulfonamides by immunochromatographic assay in eggs and chicken muscles

作　　者：李奎[1] 王喜亮[1] 金秀娥[2] 石德时[1] 熊宁[1] 郝卫芳[1] 郭延成[1] 刘群[2] 毕丁仁[1] 

机构地区：[1]华中农业大学动物医学院,武汉430070 [2]湖北省兽药监察所,武汉430070

出　　处：《中国免疫学杂志》2008年第4期336-340,共5页Chinese Journal of Immunology

基　　金：武汉市科技攻关计划项目(20022002062)

摘　　要：目的:用免疫层析试纸条法快速测定鸡蛋及鸡肉中磺胺类药物残留。方法:采用柠檬酸三钠还原法制备胶体金,用胶体金标记抗磺胺类药物母核结构单克隆抗体,磺胺类药物竞争物包被于硝酸纤维素膜,制成免疫层析快速检测试纸条。结果:该方法可以在15分钟内完成对磺胺类药物的半定量残留检测,检测磺胺间甲氧嘧啶(SMM)、磺胺对甲氧嘧啶(SMD)、磺胺二甲氧嘧啶(SDM)的灵敏度为10ng/ml,肉眼判定检测限为20ng/ml,样品中回收率在85%～95.6%之间;检测磺胺嘧啶(SDZ)的灵敏度为10ng/ml,肉眼判定检测限为40ng/ml,样品中的回收率在44.8%～60.9%之间。用本方法与高效液相色谱法(HPLC)对来自动物试验的鸡肉和鸡蛋样品进行对比检测,具有较高的符合率。结论:该方法灵敏度高,检测快速、准确,无需特殊仪器设备,可作为磺胺类药物残留的快速筛选方法。Objective：To develop a rapid immunochromatographic assay（ICA） to detect sulfonamides residue in eggs and chicken muscles.Methods：Colloidal gold was prepared through the reducing reaction by sodium citrate;Monoclonal antibody against a common structure in sulfonamides was conjugated to the colloidal gold particles as the detection reagent and N-sulfanilyl-4-aminobenzoic acid（SUL）-Human serum albumin（HSA） conjugate was immobilized to nitrocellulose membrane as the capture reagent to prepare the test strip.Results：With this method,it took only 15 min to accomplish a semi-quantitative detection for trace amount of sulfonamides,the sensitivity is 10 ng/ml,as determined with an optical density scanner.By eye measurement,the sensitivity was 20 ng/ml for sulfamonomethoine（SMM）,sulfamethoxydiazine（SMD）,sulfadimethoxine（SDM）,and 40 ng/ml for sulfadiazine（SDZ）.Based on a SMM standard curve,recoveries were from 85% to 95.6% for SMM,SMD,SDM;And from 44.8% to 60.9% for SDZ in chicken muscle and egg samples.A parallel analysis of egg and muscle samples from the animal experiment showed that the results obtained by test strips and HPLC were comparable.Conclusion：The method established is simple,sensitive,and easily hundled for detection sulfonamides residue.

关 键 词：免疫层析 试纸条 磺胺类药物 鸡肉 鸡蛋 

分 类 号：S859.84[农业科学—临床兽医学]