胃癌前病变中Fas、Fas-L表达及与幽门螺杆菌感染的关系  

The relationship between the Helicobacter pylori infection and Fas,Fas-L expression in precancerous lesions

作　　者：王立众[1] 赵占考[2] 袁红霞[3] 代二庆[2] 景秀荣[2] 张磊[2] 孙捍卫[2] 张明华[2] 

机构地区：[1]武警交通指挥部,北京100000 [2]武警医学院附属医院,天津300162 [3]天津中医药大学,天津300192

出　　处：《现代中西医结合杂志》2008年第13期1945-1946,1949,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(30371708);天津市科委应用基础研究计划项目(05YFJMJC08400)

摘　　要：目的探讨慢性萎缩性胃炎癌前病变不同病理改变胃上皮细胞的凋亡相关基因蛋白表达及与幽门螺杆菌感染的相关性。方法依胃黏膜肠化生、异型增生程度分为轻度组、中度组和中-重度组,应用快速尿素酶试验与W-S银染色法检测幽门螺杆菌感染情况,应用免疫组化SP法检测各组凋亡相关基因Fas、Fas-L的表达情况。结果中-重度组Fas基因蛋白的表达显著低于轻度组(P<0.05);中-重度组Fas-L基因蛋白的表达显著高于轻度组、中度组(P<0.01,P<0.05)。幽门螺杆菌阳性者Fas的表达显著高于幽门螺杆菌阴性者(P<0.01);中-重度组幽门螺杆菌阳性率显著低于轻度组及中度组(P<0.01)。结论癌前病变病理程度的变化与Fas、Fas-L表达变化有关,幽门螺杆菌的感染可以提高Fas的表达,Fas的表达变化可能是幽门螺杆菌相关性慢性萎缩性胃炎癌前病变细胞凋亡变化的原因之一。Objective It is to investigate wether Helicobacter pylori infection has any effects on Fas and Fas-L gene associated apoptosis, as well as its role in the different pathology of chronic atrophic gastritis accompaning lesion precancerous. Methods The patients were divided into light range group, midrange group and midst-serverity range group according to the dysplasia degree of gastric mucosa. Helicobacter pylori infection was determined with microscopic examination with Gimsa staining and the rapid urease test. S - P immunohistochemical analysis was used to detect the expression of Fas and Fas-L in Precancerous Lesions caused by H. pylori and other causes except H pylon infection. Result The positive rate of Fas protein in the midst-severity IM and（or）Dys group was significantly lower than that in the light group （P 〈 0.05）. But the expression rate of Fas-L protein in the midst-severity IM and （or）Dys was significantly higher than that in the light and the midst group （ P 〈 0.01, P 〈 0.05 ）. The positive rate of Fas protein in H. pylori（ ＋ ） lesion precancerous group was significantly higher than that in H. pylori（ - ）group （P 〈 0.01）. But the expression rate of Fas-L protein wasn＇t correlated with the H. pylori infection. The infection rate of H. pylori in the midst-severity IM and（or）Dys group was significantly higher than that in the light and the midst group （P〈 0.01 ）. Conclusion The transformation of the light to the severity in the precan- cerous lesions have relations with the changes in the expression of Fas protein and Fas - L protein. H. pylori infection could increase the expession of Fas proteinin in chronic atrophic gastritis aceompaning lesion precancerous, which may be one of the mechanism of apoptosis of gastric mucosal cell induced by H. pylori.

关 键 词：幽门螺杆菌 FAS FAS-L 慢性萎缩性胃炎 癌前病变 

分 类 号：R735.2[医药卫生—肿瘤]