紫杉醇产生菌美丽镰刀霉K178的诱变育种  

Mutagenesis and Selection of Paclitaxel-producing Fusarium mairei K178

作　　者：代文亮[1] 陶文沂[1] 程龙[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《中国医药工业杂志》2008年第4期251-253,257,共4页Chinese Journal of Pharmaceuticals

基　　金：国家自然科学基金资助项目(30370044;30470016)

摘　　要：采用紫外线与亚硝酸对美丽镰刀霉(Fusarium mairei)K178的孢子进行复合诱变,于含40μg/ml制霉菌素的平板上进行筛选,获得31株突变株,其中5株的紫杉醇摇瓶发酵产量高于出发菌株,突变株UH23的紫杉醇产量为259.8μg/L,进一步优化其发酵工艺条件,紫杉醇产量达286.4μg/L,较出发菌株提高了29.9%。Spores of paclitaxel-producing fungus Fusarium mairei K178 were mutagenized with UV radiation followed by nitrite treatment and the mutants were screened on a plate medium containing 40mg/ml nystatin. Among the thirty-one mutants obtained, five mutants exhibited an increase in paclitaxel production when fermentation was performed in shake flasks. The highest one is mutant UH23, which could produce 286.4mg/L paclitaxel under the optimized fermentation conditions, a 29.9% increase compared with the parent strain.

关 键 词：美丽镰刀霉K178 紫杉醇 复合诱变 抗性筛选 

分 类 号：Q933[生物学—微生物学]