改良四血管阻塞法建立大鼠缺血性脑损伤昏迷模型  

Modifying the four vessel occlusion to establish ischemic brain injury coma model in rats

作　　者：贾小兵[1] 叶建宁[2] 李露斯[1] 

机构地区：[1]第三军医大学西南医院神经内科,重庆400038 [2]第三军医大学新桥医院神经内科,重庆400037

出　　处：《四川医学》2008年第4期384-386,共3页Sichuan Medical Journal

基　　金：全军十一五军事医学课题基金资助项目(编码06MB237);重庆市自然科学基金资助项目(编码CSTC2006BB5091)

摘　　要：目的 制作一种较理想的大鼠缺血性脑损伤昏迷模型。方法通过用单纯四血管阻塞法和改良后的四血管阻塞法分别＋直径0．45、0．60、0．70mm注射器针头的“针控线拴法”（由我室人员周振华建立）对各组在昏迷时间和病死率之间进行比较，以便寻找一种较理想的缺血性昏迷模型。结果改良后的四血管阻塞法＋0．60mm针头“针控线拴法”能够明显延长昏迷时间可达到6．8h，并且动物不至于死亡，跟其他组别在昏迷时间上差异有统计学意义（P〈0．01）。结论改良后的四血管阻塞法＋直径0．60mm针头“针控线拴法”适合做大鼠缺血性脑损伤昏迷模型。Objective To establish a kind of ideal ischemic brain injury coma model in rats. Methods by using four vessel occlusion and modifying four vessel occlusion add diameter of 0.45mm.0.60mm.0.70mm syringe needle to make the internal carotid artery stenosis （ established by zhen hua zhou of our department） and compare the time of coma and mortality among all of the groups, to find an ideal isehemic brain injury coma model in rats. Results Modifying the four vessel occlusion add diameter 0.60mm syringe needle to make the internal carotid artery stenosis can obviously prolong the time of coma and the rat can survive,the time of coma can achieve 6 - 8 hours, compared with others groups are significant deviation（ P 〈 0.01 ） .Conclusion Modifying the four vessel occlusion add diameter 0.60mm syringe needle to make the internal carotid artery stenosis is suitable to establish ishemic brain injury coma model in rat.

关 键 词：四血管阻塞法 颈动脉狭窄 大鼠 昏迷 模型 

分 类 号：R741[医药卫生—神经病学与精神病学]