循环骤停大鼠肾集合管水通道蛋白2的表达  

Expression of renal aquaporin 2 after circulatory arrest

作　　者：王勇[1] 高林林[1] 陈卫民[1] 刘金钢[2] 

机构地区：[1]中国医科大学盛京医院麻醉科,辽宁沈阳110004 [2]中国医科大学盛京医院普通外科,辽宁沈阳110004

出　　处：《中国危重病急救医学》2008年第4期210-213,共4页Chinese Critical Care Medicine

基　　金：辽宁省教育厅科研基金资助项目（2004D282）

摘　　要：目的探讨循环骤停对肾集合管水通道蛋白2（AQP2）蛋白及基因表达的抑制作用。方法将40只大鼠随机分为两组，20只大鼠通过窒息法建立循环骤停一复苏模型（复苏组），按自主循环恢复后又分为1h和3h亚组；另20只只行机械通气但不建立窒息模型大鼠作为对照组，每组10只。分别于不同时间点取材，检测血肌酐（SCr）、尿素氮（BUN）。同时取部分肾脏，应用蛋白质免疫印迹法（Western blotting）及逆转录-聚合酶链反应（RT—PCR）分别检测AQP2蛋白及基因表达。结果各组动物复苏时间、SCr、BUN比较差异无统计学意义。复苏后1h和3hAQP2蛋白及mRNA表达较对照组相应时间点明显下降（蛋白表达：1h为38．35±2．08比41．06±1．04，3h为31．89±1．57比41．45v0．58；mRNA表达：1h为0．61±0．13比0．87±0．14，3h为0．54±0．11比0．85±0．12，P均d0．01），且复苏后3hAQP2蛋白及mRNA表达较1h进一步下降（P均d0．01）。结论循环骤停会导致肾AQP2蛋白、基因表达下调，这一改变早于SCr、BUN改变。Objective To investigate the inhibition of renal aquaporin 2 （AQP2） and AQP2 mRNA expression due to circulatory arrest. Methods Forty rats were randomized into two groups. The circulatory arrest-resuscitation model was reproduced in resuscitation group （20 rats）. Rats in control group were subjected to the same proceduce hut only received mechanical ventilation without asphyxia and resuscitation （20 rats）. Rats in each group were divided into 1-hour group and 3-hour group randomly （10 rats in each group）. Serum creatinine （SCr） and blood urine nitrogen （BUN） concentrations were determined in every rat. Medulla of kidney in every rat was isolated for the determination of AQP2 and AQP2 mRNA expression with Western blotting and reverse transcription-polymerase chain reaction （RT-PCR）. Results There were no differences in resuscitation time, SCr and BUN concentrations between resuscitation and control groups. The AQP2 protein and mRNA expression in resuscitation 1-hour and 3-hour groups were significantly decreased compared with those in control group at the same time points [protein： 38.35±2. 08 vs. 41.06± 1.04 （1-hour）, 31.89±1.57 vs. 41.45±0.58 （3-hour）; mRNA： 0.61±0.13 vs. 0.87±0.14 （1-hour）, 0. 54±0.11 vs. 0. 85±0.12 （3-hour）, all P〈0. 019, and they were further lowered in resuscitation 3-hour group （both P〈0. 01）. Conclusion Circulatory arrest would lead to down regulation of renal AQP2 and AQP2 mRNA expression. These changes appear earlier than those of SCr and BUN.

关 键 词：循环骤停 水通道蛋白2 心肺复苏 

分 类 号：R459.7[医药卫生—急诊医学]