转化生长因子-β和胰岛素对人牙周膜细胞增殖的影响  

The effects of transforming growth factor β and insulin on proliferation of human periodontal ligament cells

作　　者：吴织芬[1] 董广英[1] 万玲[1] 

机构地区：[1]第四军医大学口腔医学院

出　　处：《中华口腔医学杂志》1997年第5期300-302,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金

摘　　要：为了评价转化生长因子－β（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒ－β，ＴＧＦ－β）和胰岛素单独或联合应用，对人牙周膜（ｐｅｒｉｏｄｏｎｔａｌｌｉｇａｍｅｎｔ，ＰＤＬ）细胞增殖的影响，并以两者的最佳效应浓度观察其作用的时间效应。作者采用体外细胞培养技术和ＭＴＴ［３－（４，５－ｄｉｍｅｔｈｙｌｔｈｉａｚｏｌ－２－ｙｌ）－２，５－ｄｉｐｈｅｎｙｌｔｅｔｒａ－ｚｏｌｉｕｍｂｒｏｍｉｄｅ］比色法进行观察。结果：ＴＧＦ－β浓度在０．１～１０μｇ／Ｌ、胰岛素浓度在１０～１０００Ｕ／Ｌ时，对人ＰＤＬ细胞有非常显著的促增殖作用（Ｐ＜０．０１），从第３天起增殖最为显著（Ｐ＜０．０１），并持续到第５天以后。两者的联合应用较单独用时作用显著（Ｐ＜０．０１）。结论：在牙周组织引导性再生技术中，若能同时结合ＴＧＦ－β和胰岛素的应用，可望提高治疗的效果。Aim: To evaluate biological effects in vitro of transforming growth factor β (TGF β) and /or insulin on proliferation of the human periodontal ligament (PDL) cells. Methods: Cell cultures and MTT [3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium bromide] colorimetric assay. Result: TGF β at concentrations of 0.1 to 10 μg/L and insulin at 10 to 1 000 U/L had significant proliferative effects on human PDL cells ( P <0.01). From the 3rd day, the significant proliferation of PDL cells by TGF β or insulin were observed ( P <0.01) and lasted to the 5th day. The effect of combination of TGF β with insulin was the most significant one ( P <0.01). Conclusion: TGF β and/or insulin may be valuable in promoting the regeneration of periodontal connective tissue.

关 键 词：转化生长因子 胰岛素 牙周膜细胞 牙周病 

分 类 号：R781.4[医药卫生—口腔医学]