地方品种固始鸭与樱桃谷鸭α-干扰素基因的克隆与遗传进化分析  

Cloning and Genetic Analysis of Interferon-α Gene in Gushi Duck and Yingtaogu Duck of Local Breed

作　　者：管倩[1] 崔保安[1] 陈红英[1] 杨明凡[1] 郭小参[1] 吕晓丽[1] 王东方[1] 

机构地区：[1]河南农业大学牧医工程学院

出　　处：《华南农业大学学报》2008年第2期104-107,共4页Journal of South China Agricultural University

基　　金：“十五”国家食品安全重大攻关专项(2001BA804A30-11)

摘　　要：根据GenBank发表的鸭α-干扰素(interferon-alpha,IFN-α)基因序列(AY879230),设计并合成了1对引物,提取固始鸭和樱桃谷鸭基因组DNA,应用PCR技术扩增地方品种固始鸭和樱桃谷鸭IFN-α基因,并克隆、测序.测序结果表明获得了固始鸭和樱桃谷鸭IFN-α基因,大小均为584 bp,包含1个IFN-α基因完整的开放阅读框,编码191个氨基酸的多肽.推导的氨基酸有2个潜在的N-糖基化位点,有7个与二硫键形成有关的半胱氨酸.克隆的固始鸭和樱桃谷鸭IFN-α基因与北京鸭IFN-α基因的氨基酸序列比较,仅固始鸭IFN-α基因发生突变D38N.固始鸭和樱桃谷鸭IFN-α基因与其他IFN-α基因的氨基酸序列遗传进化树分析表明樱桃谷鸭与北京鸭有很近的亲缘关系,固始鸭次之.地方品种固始鸭在非常保守的38位发生了氨基酸突变,推测这可能与该品种鸭具有很强的抗病能力有关.One pair of primers was designed and synthesized according to the duck interferon-α （IFN-α） gene nucleotide sequence（AY879230） in GenBank. Genome DNA was extracted from Gushi duck （GS duck） and Yingtaogu duck （YT duck）, and then the IFN-α gene fragment was amplified from both GS duck and YT cluck by PCR and the amplicons were cloned and sequenced. The results indicated that the nucleotide sequences of IFN-α genes from both duck breeds were 584 bp, including one open-reading frame, encoding 191 amino acid polypeptide. Both of the deduced amino acid sequences contained two potential N-glycosylafion sites and seven cysteines which are related to shape disulfide bond. Compared to the amino acid sequence of Beijing duck （BJ duck）IFN-α gene, GS duck IFN-α gene show D^38N mutation at 38 site amino acid. Phylogenetic analysis showed that there were close relationships between YT duck and BJ duck. mutation was found at the amino acid sequence position 38 of local breed of GS duck, which was highly conserved usually ,and it is speculated that this may be related to its high resistance against diseases.

关 键 词：鸭 Α-干扰素 克隆 遗传进化分析 

分 类 号：S858.32[农业科学—临床兽医学]