庆大霉素对组织培养角膜细胞毒性的影响  

The toxicity of gentamicin on corneal cells in culture

作　　者：康凤英[1] 祝寿荣[1] 张圣明[1] 李兴英[1] 张浩润 

机构地区：[1]山东省潍坊医学院眼科学教研室

出　　处：《中华眼科杂志》1997年第5期366-369,共4页Chinese Journal of Ophthalmology

基　　金：山东省卫生厅基金

摘　　要：目的探讨临床眼部应用庆大霉素的安全剂量。方法采用体外培养的人角膜上皮细胞、内皮细胞、基质细胞分别在生长旺盛形成单层时加入不同浓度的庆大霉素，于不同时相在光镜和扫描电镜下观察细胞形态。结果浓度为１ｍｇ／ｍｌ不含保存剂的庆大霉素液对培养的３种细胞作用４８小时无损害；当药物浓度增加到２ｍｇ／ｍｌ和４ｍｇ／ｍｌ时，光镜下显示，不同的角膜细胞在不同时相均出现细胞病变，其中以内皮细胞损害最为严重，其次是上皮细胞，基质细胞损害最轻，损害程度与庆大霉素浓度的增加和作用时间的延长有关。结论临床眼部应用庆大霉素的安全剂量为１ｍｇ／ｍｌ。Objective To determine the safe local concentration of gentamicin. Methods The authors studied the toxicity of gentamicin (no preservative) on 3 types of human corneal cell in culture with different concentrations. Results There was no toxic effect on the corneal epithelium, stroma and endothelium with gentamicin 1 mg. per milliliter in 48 h. in vitro, while the concentration was up to 2 mg. or 4 mg. per milliliter, all types of corneal cells appeared cytopathic effect (CPE), the toxicity of gentamicin to the corneal cells is in direct proportion to its concentration and time term of incubation, and the toxicity was the greatest on endothelium, slightest on stroma. Conclusion It is suggested that the safe dosage of local concentration of gentamicin be 1 mg. per milliliter in human eyes.

关 键 词：角膜细胞 庆大霉素 毒性 人 

分 类 号：R772.2[医药卫生—眼科] R978.12[医药卫生—临床医学]