MnSOD模拟化合物诱导人白血病多药耐药K562/ADM细胞凋亡  

Apoptosis of K562/ADM cells induced by mimics of MnSOD

作　　者：安娴[1] 魏虎来[1] 祁萍[1] 窦伟[2] 刘伟生[2] 

机构地区：[1]兰州大学医学院实验中心,甘肃兰州730000 [2]兰州大学化学化工学院,甘肃兰州730000

出　　处：《现代肿瘤医学》2008年第5期703-706,共4页Journal of Modern Oncology

摘　　要：目的:研究锰超氧化物岐化酶模拟化合物(mimics of manganese superoxi dedismutase,MnSODm)对人白血病K562/ADM耐药细胞增殖抑制及诱导凋亡作用。方法:以人白血病高表达耐药基因(mdr1)的K562/ADM多药耐药细胞为靶细胞,应用四氮唑蓝比色法(MTT法)测定细胞增殖活性;光学显微镜、荧光显微镜和透射电镜观察细胞形态学变化;Annexin V/PI双标记法检测K562/ADM细胞凋亡。结果:(1-50)mg/LMn-SODm可抑制K562/ADM细胞增殖(P<0.01),并呈时间和浓度依赖性。MnSODm诱导48h后,K562/ADM细胞出现典型的凋亡形态学改变;Annexin V/PI双染显示凋亡细胞显著增高。结论:MnSODm体外明显抑制人白血病K562/ADM耐药细胞的增殖,并诱导K562/ADM细胞凋亡。Objective：To investigate the proliferation - inhibiting and apoptosis - inducing activity of mimics of manganese superoxide dismutase （MnSODm） on human muhidrug - resistant leukemia cell line K562/ADM in vitro. Methods： Human muhidrug - resistant leukemia cell line K562/ADM over - expressing mdrl gene was used as the target cells. The cell proliferating activity was assessed with a MTT colorimetric assay. The morphology of K562/ ADM cells was observed with optical, fluorescent and electronic microscopy, and apoptosis was explored by double staining of FTTC - Annexin V and propidium iodide （PI）. Results ： MnSODm （ 1 - 50 mg/L） obviously inhibited the proliferation of K562/ADM cells in a manner depending on both dose and time （ P 〈 0.01 ）. The cells treated with MnSODm showed the typical apoptotic morphological changes. The apoptotic rate of MnSODm - treated K562/ADM cells assed with Annexin V/PI staining was greatly increased from 3.32% to 13.91% and 55.0% after exposure to 10 mg/L and 50 mg/L for 48 hours. Conclusion： MnSODm greatly inhibits the proliferation of drug - resistant K562/ADM cells, and induces apoptosis of K562/ADM cells in vitro.

关 键 词：MnSOD模拟化合物 白血病 多药耐药 抗肿瘤活性 凋亡 

分 类 号：R73-3[医药卫生—肿瘤] R737.7[医药卫生—临床医学]