缺氧诱导体外培养人视网膜色素上皮细胞凋亡  

Hypoxia induces apoptosis of the cultured human retinal pigment epithelial cells

作　　者：刘敏[1] 王雨生[1] 赵炜[1] 朱洁[1] 李夏[1] 杨秀梅[1] 

机构地区：[1]第四军医大学西京医院眼科全军眼科研究所,中国陕西省西安市710032

出　　处：《国际眼科杂志》2008年第4期679-682,共4页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30371516;30672291);中国教育部留学回国人员科研启动基金资助项目(2004)~~

摘　　要：目的:了解缺氧条件下体外培养人视网膜色素上皮(retinal pigment epithelium,RPE)细胞的凋亡情况。方法:将培养的人RPE细胞置于含10mL/LO2、50mL/LCO2和940mL/LN2的培养箱内建立缺氧模型。于缺氧后1,3,6,12,24h,利用扫描电镜、透射电镜、脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal-deoxynucle-otidyl transferase mediated nick end labeling,TUNEL)技术和流式细胞仪测定RPE细胞凋亡水平。结果:常氧状态下RPE细胞生长良好,几乎没有凋亡(TUNEL法测凋亡指数为1.2)。缺氧条件下,RPE细胞发生了不同程度的凋亡,缺氧后3h凋亡水平达峰值(凋亡指数为34.43)。结论:缺氧可导致体外培养的人RPE细胞凋亡,提示RPE凋亡可能参与了某些缺血缺氧性眼病的发生。AIM：To evaluate the effect of hypoxia on apoptosis of cultured human retinal pigment epithelial（RPE）cells.METHODS：In the hypoxia model,human REP cells were cultured in a special incubator containing volume fraction of 10mL/L O2,50mL/L CO2 and 940mL/L N2 for 0 hour,1 hour,3,6,12 and 24 hours.The level of apoptosis was individually measured with scanning and transmis-sion electron microscopy,terminal-deoxynucleotidyl transferase mediated nick end labeling（TUNEL）and flow cytometry.RESULTS：RPE cells grew well under normoxic conditions.The apoptosis was significantly activated and peaked at 3 hours in the cultured RPE cells under hypoxia.CONCLUSION：Hypoxia can induce apoptosis in the cultured RPE cells,suggesting that RPE apoptosis might play a role in ischemic ocular diseases.

关 键 词：视网膜色素上皮细胞 缺氧 凋亡 

分 类 号：R774.1[医药卫生—眼科]