TGF-β2对体外培养牛晶状体上皮细胞CTGF蛋白及mRNA表达的影响  

Effect of transforming growth factor-β_2 on expression of connective tissue growth factor mRNA and protein in cultured bovine lens epithelial cells

作　　者：陈博[1] 曹阳[1] 笪邦红[2] 胡义珍[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,中国湖北省武汉市430022 [2]华中科技大学同济医学院附属协和医院急诊科,中国湖北省武汉市430022

出　　处：《国际眼科杂志》2008年第4期701-704,共4页International Eye Science

基　　金：中国湖北省自然科学基金资助项目(No.2006ABA097)~~

摘　　要：目的：观察转化生长因子-β2（transforming growth factor-β2，TGF-β2）对体外培养牛眼晶状体上皮细胞（lens epithelial cell，LEC）结缔组织生长因子（connective tissue growth factor，CTGF）mRNA和蛋白表达的影响。方法：将体外培养2-3代牛LEC分为对照组和实验组，实验组分别经1，5，10μg/LTGF-β2处理24h后，采用半定量RT-PCR和Westernblot技术检测CTGF mRNA以及蛋白表达的变化。结果：与对照组相比，1μg/LTGF-β2组对牛LECCTGF/β-actin吸光度比值和CTGF/β-actin灰度比值的表达无明显影响（P〉0.05），5，10μg/LTGF-β2组可以明显上调RT-PCR和Western blot中CTGF/β-actin比值（P〈0.01）。结论：TGF-β2可促进体外培养牛LEC表达CTGF蛋白及mRNA。AIM：To observe the effects of transforming growth factor-β2（TGF-β2）on the mRNA and protein expression of connective tissue growth factor（CTGF）in bovine lens epithelial cells（LEC）.METHODS：Cultured 2-3 passage bovine LEC in vitro were divided into control group and experimental group treated with 1,5,10μg/L TGF-β2 for 24 hours respectively.The mRNA and protein of CTGF in bovine LEC were examined respectively by semiquantitative RT-PCR and Western blot,and the expression levels of CTGF were determined relative to that of β-actin.RESULTS：In comparison with control bovine LEC,1μg/L TGF-β2 did not affect the values of CTGF/β-actin both in RT-PCR and Western blot（P〉0.05）.However,5,10μg/L TGF-β2 significantly up-regulated the values of CTGF/β-actin in the examinations（P〈0.01）.CONCLUSION：The data suggested that TGF-β2 could promote the expression of CTGF at mRNA and protein level obviously in bovine LEC.The role of CTGF in LEC transdifferentiation induced by TGF-β2 in pathogenesis of posterior capsule opacification is worthy of further investigation.

关 键 词：转化生长因子 晶状体上皮细胞 结缔组织生长因子 白内障 

分 类 号：R776.1[医药卫生—眼科] R977.12[医药卫生—临床医学]