他克莫司发酵培养基的响应面优化设计  

Optimization of medium components for tacrolimus production using response surface design

作　　者：顾骏[1] 叶蕊芳[1] 郑黎[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《工业微生物》2008年第2期60-63,共4页Industrial Microbiology

摘　　要：用两水平因子设计考察培养基中的所有七个成分对他克莫司产量的影响大小,选出其中影响较大的葡萄糖、酵母粉、花生粉和NaCl进行最佳浓度范围考察,然后用中心组合方法及响应面分析确定最佳配比,他克莫司的产量提高了55%左右。The two-level factorial design was employed to evaluate the influence of all the seven factors on the tacrolimus production. The optimal concentration ranges of the glucose, yeast extract, peanuts powder and NaCl were investigated, which obviously influenced the tacrolimus production, and the medium was optimized by using the central composite design and response surface analysis. As a result, the production of tacrolimus was increased by 55%.

关 键 词：他克莫司 中心组合设计 发酵 

分 类 号：TQ465[化学工程—制药化工]