固定化球形红细菌去除镉的动力学及其与质粒的关系  

Kinetics of Cadmium Removal by Immobilized Rhodobacter sphaeroides and Its Relation with Plasmid

作　　者：白红娟[1] 张肇铭[1] 李保珍[1] 杨官娥[1] 李琦[1] 

机构地区：[1]山西大学生命科学与技术学院

出　　处：《应用与环境生物学报》2008年第2期249-252,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技攻关计划项目(No.2001BA540C)资助~~

摘　　要：利用聚乙烯醇为载体包埋固定化球形红细菌，研究了固定化微生物去除Cd^2＋的动力学特征，结果表明，在Cd^2＋初始浓度为20～160mg／L时，固定化球形红细菌具有良好的去除能力，去除率可达75％以上，固定化球形红细菌去除Cd^2＋的反应遵循二级反应动力学方程-dC／dt=k2C^2＋k0,C＋k0，R^2=0．9577—0．9938，随着Cd^2＋初始浓度的增加，固定化球形红细菌对Cd^2＋的最大去除速率P下降，半衰期T1/2增加，抑制作用增强，此外，通过质粒检测，该菌株含有一个较大质粒，抗镉和去除镉的基因均位于质粒上。Rhodobacter sphaeroides was acclimated and immobilized in polyvinylalcohol gel beads, and the immobilized R. sphaeroides was used to remove Cd^2＋ from aqueous solution. The kinetic characteristics of Cd^2＋ removal by the immobilized R. sphaeroides were studied. The experimental results demonstrated that the immobilized R. sphaeroides was effective in removing Cd^2＋ from aqueous solution, and the removal rate could reach more than 75% in presence of 20 - 160 mg/L of cadmium. The kinetics of Cd^2＋ removal by the immobilized R. sphaeroides followed pseudo-second order equation - dC/dt = k2 C^2 ＋ k1C ＋ k0 , and the correlation coefficient values R^2 = 0. 957 7 - 0. 993 8. The maximum removal rates p of Cd^2＋ decreased while the values of half life Tl/2 prolonged, and inhibition effect of Cd^2＋ increased greatly with the increasing of initial concentration of Cd^2＋. Also, the strain had a big plasmid, and its Cd-resistance and Cd-removal genes were located on the plasmid. Fig 3, Tab 1, Ref 19

关 键 词：固定化 球形红细菌 镉 动力学 质粒 

分 类 号：X172[环境科学与工程—环境科学]