Iressa抑制胃癌SGC-7901细胞生长的研究  

Experimental study on iressa inhibits the proliferation of gastric cancer SGC-7901 cells

作　　者：姚煜[1] 田涛[1] 李春丽[1] 锁爱莉[1] 南克俊[1] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤内科,西安710061

出　　处：《陕西医学杂志》2008年第4期459-461,共3页Shaanxi Medical Journal

基　　金：陕西省攻关项目[2006K09-G6-(1)]

摘　　要：目的：探讨iressa对胃癌SGC.7901细胞增殖，细胞周期和细胞凋亡的影响。方法：用四氮甲唑蓝（MTT）法检测不同浓度iressa对胃癌SGC-7901细胞增殖的影响；用流式细胞仪检测不同浓度iressa对胃癌SGC-7901细胞周期分布和细胞凋亡的影响。结果：在5～20μmol／L浓度范围内，iressa对SGC-7901的生长具有明显的抑制作用，并呈时间和剂量依赖性。10μmol／L和20uol／L的iressa可以导致SGC-7901细胞G1期阻滞，并引起细胞凋亡。结论：Iressa可改变SGC-7901细胞周期分布，诱导细胞凋亡，从而抑制细胞增殖。Objectives ：To investigate the effect of iressa on the proliferation,cell cycle and apoptosis of human gastric cancer SGC-7901 ceils. Methods：The proliferation inhibition of SGC-790 1 cells exposed to different concentration of iressa was observed by MTT assay. The cell cycle and apoptosis of SGC-7901 cells exposed to different concentration of iressa was analyzed by flowcytometry. Results：Within the rang of 5-20 umol/L,iressa obviously inhibited the proliferation of SGC-790 1 cells in a time--and dose--dependent manner. 10umol/L and20 umol/L iressa caused SGC-790 1 cells to undergo G1 arrest and induced apoptosis. Conclusion： Iressa could inhibited SGC-7901 cells proliferation,caused G1 arrest and induced apoptosis.

关 键 词：胃肿瘤/免疫学 苯胺化合物/药理学 细胞凋亡/免疫学 细胞周期 细胞分裂 @SGC-7901细胞 受体 表皮生长因子 

分 类 号：R735.2[医药卫生—肿瘤] R734.2[医药卫生—临床医学]