利用“永久 F_2”群体定位抗赤霉病 QTL  

Mapping QTLs Associated with Resistance to Fusarium Head Blight Using an"Immortalized F_2"Population

作　　者：田大刚[1] 林 峰[1] 张彩琴[1] 张政值[1] 薛树林[1] 曹 勇[1] 李春军[1] 马正强[1] 

机构地区：[1]应用植物基因组学实验室和南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《作物学报》2008年第4期539-544,共6页Acta Agronomica Sinica

基　　金：国家基础研究发展计划(973 计划)项目(2006CB101702);国家自然科学基金项目(30430440; 30025030);国家高技术研究发展计划 (863 计划)项目(2003AA207100)

摘　　要：为了研究抗赤霉病侵染性的遗传, 利用感赤霉病品种南大 2419 和抗赤霉病品种望水白杂交单粒传获得的重组自交系群体 132 个株系间的随机配对组合, 构建了一个包含 198 个株系的"永久 F2"群体。通过两年抗侵染田间试验和 QTL 作图, 定位了 6 个抗侵染 QTL, 其中抗性等位位点源于望水白的 Qfhi.nau-4B 和 Qfhi.nau-5A 以及源于南大2419 的 Qfhi.nau-2B 的效应较为稳定。Qfhi.nau-4B 和 Qfhi.nau-5A 的效应较大且以加性效应为主, 前者存在部分显性基因效应。此外, 还检测到 4 对显著的互作位点。这些结果进一步说明赤霉病抗性遗传的复杂性, 同时也表明在利用望水白进行抗赤霉病育种时早代选择抗侵染性是可行的。Fusarium head blight （FHB） or scab disease, caused by Gibberrella zeae （Schw.） Petch, is a serious disease in many wheat （Triticum aestivum L.） growing regions worldwide. To study the inheritance of scab resistance against fungal penetration （type Ⅰ resistance）, an 198 lines ＂immortalized F2＂population was constructed by crossing between recombinant inbred lines chosen by random permutation of 132 RILs from the RIL population derived from the cross between scab-susceptible cultivar Nanda 2419 and scab-resistant cultivar Wangshuibai. The population were then evaluated for percentage of infected spikes （PIS） across two years and six chromosome regions were detected for their association with type Ⅰ resistance through interval mapping, among which Qfhi.nau-4B and Qfhi.nau-5A with the resistance alleles originated from Wangshuibai and Qfhi.nau-2B with the resistance allele from Nanda 2419 were consistently detected. Qfhi.nau-4B and Qfhi.nau-5A had the largest effects among the detected QTLs and both showed mainly the additive allelic effects, the former also had partial dominance. In addition, four pairs of significant interaction loci were identified. These results further demonstrate that wheat scab resistance are under complex genetic control and also imply that early generation selection for type Ⅰ resistance in scab resistance breeding is feasible with Wangshuibai as the parent.

关 键 词：赤霉病 望水白 “永久F2”群体 类型Ⅰ抗性 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]