硫化氢下调大鼠血小板L-精氨酸/一氧化氮合酶/一氧化氮通路  

Hydrogen sulfide down-regulates L-arginine/nitric oxide synthase/NO pathway in platelets of rat

作　　者：冯浩楼[1] 崔玉英[1] 魏芳[1] 张明艳[1] 赵燕燕[1] 李军[1] 

机构地区：[1]河北大学医学部生理教研室,河北保定071000

出　　处：《中国现代医学杂志》2008年第7期852-855,859,共5页China Journal of Modern Medicine

基　　金：保定市科技攻关课题(06F04-1);河北省科学技术研究与发展计划项目(06276121)

摘　　要：目的观察硫化氢(hydrogen sulfide,H2S)对血小板L-精氨酸/一氧化氮合酶/一氧化氮(L-Arg/NOS/NO)通路的影响以探讨H2S和NO这两种气体信号分子间的相互作用。方法离体孵育大鼠血小板,加入H2S气体溶液(10-6～10-3mol/L)孵育2h,采用Greiss法测定血小板孵育液亚硝酸盐(NO2-)含量;同位素示踪法检测血小板NOS活性及L-Arg转运。结果一次性给予H2S(10-6～10-3mol/L)孵育2h,呈浓度依赖的抑制了L-Arg/NO通路,血小板孵育液中NO2-含量比对照组分别降低6%、11%(均P>0.05)、24%、36%和52%(均P<0.01);NOS活性分别下降1%、14%(P>0.05)、25%、37%和42%(P<0.01);L-Arg转运分别减少12%(P>0.05)到72%(P<0.01);IC50分别为53.02、41.63及21.53μmol/L(P<0.01)。结论H2S通过抑制血小板L-Arg转运和NOS活性,下调L-Arg/NOS/NO通路,从而减少血小板NO生成。[Objective] To observe the influence of hydrogen sulfide （H S） on L-arginine/nitric oxide synthase （NOS）/nitric oxide （NO） pathway in rat platelets, and explore the interaction between H2S and NO as cardiovascular regulatory gasotransmitters. [Methods] Washed platelets （ 1 ×10^8 pt/mL） was incubated with H2S （10^-6 mol/L-10^-3 mol/L） for 2 h, the nitrite production was measured by Greiss assay, NOS activities and L-arginine transport rate were determined by isotope tracer method. [Results] After the incubation for 2 h, H2S （10^-6 mol/L-10^-3 tool/L） inhibited the L-arginine/NOS/NO pathway in rat platelets in a dose-dependent manner, the nitrite production of platelets decreased by 6%, 11%（P〉0.05）, 24%, 36% and 52%（P〈0.01）, NOS activity reduced by 1%,14%（P〉0.05）, 25%, 37% and 42%（P〈0.01）, L-arginine transportation decreased by 12%（P〉0.05）to 72%（P〈0.01） and their IC50 were 53.02 μmol/L, 41.63μmol/L and 21.53μmol/L（P 〈0.01）, respectively. [Conclusion] H2S inhibits NO release by inhibiting L-arginine transport and NOS activity, down-regulating L-arginine/NOS/NO pathway in rat platelets.

关 键 词：血小板 硫化氢 一氧化氮 一氧化氮舍酶 L-精氨酸转运 

分 类 号：R363.2[医药卫生—病理学] R631.4[医药卫生—基础医学]