龙眼组培的褐变抑制研究  

Study on the Browning-inhibition of Longan in Tissue Culture

作　　者：曹鸿斌[1] 刘魁英[1] 赵宗芸[1] 黄米丽[1] 

机构地区：[1]广东海洋大学农学院,广东湛江524088

出　　处：《安徽农业科学》2008年第8期3132-3133,共2页Journal of Anhui Agricultural Sciences

基　　金：广东海洋大学资助项目

摘　　要：[目的]探求有效抑制龙眼组培时外植体褐变的方法。[方法]以龙眼嫩梢茎段为材料,采用多种抗褐变方法,筛选对龙眼组织培养中的外植体褐变抑制效果最好的方法。[结果]采用6.00g/LPVP溶液进行预处理对龙眼外植体褐变的抑制效果最好。当6-BA浓度为1.92～1.93mg/L、KT浓度为0.88～1.93mg/L、NAA浓度为0.05～0.06mg/L时,外植体的褐变程度最轻。添加2.00g/L的PVP和活性炭的培养基对外植体褐变的抑制效果最好。[结论]选取培养基MS+NAA0.05～0.06mg/L+6-BA1.92～1.93mg/L+KT0.88～1.93mg/L+PVP2.00g/L,结合6.00g/LPVP溶液的预处理,对龙眼组织培养时外植体褐变的抑制效果最好。[Objective] The aim was to seek for the effective method of inhibiting the browning of Longan explants in tissue culture. [Method] With stem segments of tender Longan shoot as material, many methods of resisting browning were used to screen out the method with best inhibition effect on the browning of Longan explants in tissue culture. [Result] The inhibition effect on the browning of Longan explants pretreated with 6.00 g/LPVP solution was best. When the 6-BA was 1.92-1.93 mg/L, KT was 0.88-1.93 mg/L and NAA was 0.05-0.06 mg/L, the browning degree of explants was lightest. The inhibition effect of medium with 2.0 g/L PVP and active carbon on the browning of explants was best. [Conclusion] The combination of medium MS＋NAA 0.05-0.06 mg/L＋6-BA 1.92-1.93 mg/L＋KT 0.88-1.93 mg/L＋PVP 2.00 g/L and pretreatment with 6.00 g/L PVP solution had the best inhibition effect on the browning of Longan explants in tissue culture.

关 键 词：龙眼 组培 褐变 

分 类 号：Q943.1[生物学—植物学]