离子注入对桉树苗期叶性状的影响  

Studies on Effect of Ion Implantation on Leaf Traits of Eucalyptus during Seedling Stage

作　　者：李光友[1] 徐建民[1] 白嘉雨[1] 陆钊华[1] 王伟[1] 陈儒香[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《安徽农业科学》2008年第8期3223-3225,3261,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省自然基金资助项目(05004910);"十一五"国家科技攻关项目子课题(2006BAD01A15);中国林业科学院热带林业研究所中央级公益性科研院所基本业务费资助项目(200708)

摘　　要：[目的]为离子注入生物工程在树木改良研究上提供新的途径。[方法]按1014和1015N+/cm22种剂量处理分别对16个桉树家系的种子进行离子注入,观察注入后苗木的生长情况。[结果]不同家系叶样的湿、干重均达到极显著差异,不同处理的叶干重达到显著差异;桉树的叶长、宽和叶面积在各家系之间达到极显著差异;4个叶指标(叶体积、叶内体积、叶比重和空隙率)在家系和处理间均达到极显著差异;4个叶生理指标除单株叶含水率LWC在处理间差异不明显外,其他(RWC、RWD和EC)均达到极显著差异,说明不同家系的生理状况和抗性能力有差异。[结论]离子注入可引起桉树叶结构的变化;通过选择和离子注入处理可获得抗性强的桉树家系/无性系。[Objective] The research aimed to provide new approach for study on tree improvement through ion implantation bioengineering. [Method] The seeds of 16 families of Eucalyptus were implanted N＋ ions at two doses of 10^14 and 10^15N^＋/cm^2, resp, and the growth status of the seedling were observed after ion implantation. [Result] The wet and the dry weight of leaf sample all had extremely significant differences among families and dry weight of leaf had extremely significant differences among treatments. The leaf length and the width and leaf area had extremely significant differences among families. 4 leaf indexes including the leaf Volume, the inside Volume, the specific gravity, the ratio of interspaces all reached extremely significant differences among families and between treatments. 4 physiological index of leaf including R WC （relative water content）, RWD （relative water deficit）, EC （relative electricity conductance） and LWC （leaf water content per tree） all reached extremely significant differences expect that LWC had little difference between treatments, showing that there were some difference on physiological status and resistance ability among families. [Conclusion] The ion implantation could induce the change of leaf structure and the elite families/clones can be selected through selection and ion implantation.

关 键 词：离子注入 桉树 苗期生长 叶性状 

分 类 号：S718.43[农业科学—林学]