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机构地区:[1]山西大学生命科学与技术学院,山西太原030006 [2]山西大学生物技术研究所,山西太原030006
出 处:《安徽农业科学》2008年第8期3327-3328,共2页Journal of Anhui Agricultural Sciences
基 金:山西省科技厅项目(编号:2006031195)
摘 要:[目的]以玉米秸粉为原料生产微生物饲料。[方法]采用L16(45)正交试验设计,利用多株微生物固态混合发酵,研究以玉米秸粉为原料生产微生物饲料的工艺。[结果]菌种配比、原料初始pH值、原料C/N和发酵时间都会影响发酵产物中真蛋白含量和粗纤维含量。试验所得的最优条件为:初始pH值6.0,硫酸铵添加量为3.0%,接种量为黑曲霉H64%,木霉M32%,酵母Y31%、Y21%、Y261%,发酵时间60h。在该最优条件下,发酵产物中真蛋白含量10.1%,增加了6.0%;粗纤维含量30.2%,减少了6.9%;蛋白酶含量131U/g,糖化酶含量280U/g。[结论]以玉米秸粉为原料生产微生物饲料是一种很好的资源利用方式。[Objective] The research aimed to produce foodstuff with fermented stem powder of com.[Method] Stem powder of corn was fermented to get foodstuff by using some strains and L16 (4^5)orthogonal design. [Result] The fermentation time, stains ratio, pH value and ratio of C and N could effect the contents of quantity of true protein and crude fiber of fermentation products. The optimum fermentation conditions were pH value 6.0, ammonium sulfate 3.0%, inoculating quantity of Aspergillus niger V. Tiegh H6 4%, Trichoderma sp. M3 2%, yeast Y3 1%, Y2 1%, Y36 1%, fermentation time 60 h, respectively. Under these conditions, The fermentation products contained true protein 10.1% that was increased 6.0% compared with original content, crude fiber 30.2% that was decreased 6.9% compared with original content, proteinase 131 U/g, saccarogenic amylase 280 U/g. [Conclusion] It was a good way of using resource by producing foodstuff with fermented stem powder of corn.
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