维生素C对中华绒螯蟹非特异性免疫的影响  

Effect of dietary vitamin C on the non-specific immunity of Eriocheir sinensis

作　　者：艾春香[1] 陈立侨[1] 刘晓玲[1] 高露姣[1] 温小波[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《水产学报》2008年第2期249-256,共8页Journal of Fisheries of China

基　　金：国家自然科学基金(30271012);高等学校博士点专项基金(2000026903);教育部回国留学人员科研启动基金

摘　　要：通过在以鱼粉、豆粕、玉米粉和麦麸为主要原料配制而成的每100g基础饲料中分别添加0、250、500、1000、1500mg的Vc（以Vc多聚磷酸酯为Vc源）配制5种试验饲料，对均重为（37．52±2．19）g的中华绒螯蟹进行为期60d的饲养试验，以酚氧化酶（PO）、抗菌力（Ua）、溶菌酶（Ua）、超氧化物歧化酶（SOD）、碱性磷酸酶（AKP）和酸性磷酸酶（ACP）等指标为判据，研究Vf对中华绒螯蟹体液免疫因子的影响。结果表明，Vc对中华绒螯蟹各组织器官中PO、Ua、UL、SOD、AKP和ACP等活性有显著或极显著的影响（P〈0．05或P〈0．01），在适宜范围内，随着Vc添加量的增加，PO、U。uL、AKP和ACP活性显著增强，从而增强中华绒螯蟹的非特异性免疫力，而SOD活性则显著降低（P〈0．05），表明Vc作为天然抗氧化剂在中华绒螯蟹体内发挥了很大的作用。综上所述，Vc作为免疫刺激剂能有效地增强中华绒螯蟹的非特异性免疫力，且Vc适宜添加量以500～1000mg·（100g）^-1饲料为宜。The studies were conducted to determine the effects of Vc on the immune factors of the Chinese mitten- handed crabs Eriocheir sinensis[initial mean weight of （37.52±2.29） g]. The crabs were distributed into the glass tanks of 200 L capacity using a completely randomized design with five treatments respectively and each treatment was stocked with 10 crabs and was run in triplicate per treatment. The crabs E. sinensis were fed 60 d with a series of test diets containing graded levels of Vc [ 0, 250, 500, 1000, 1500 mg·（100g）^- 1 diet respectively]. Each treatment was run in triplicate. The activities of phenoloxidase （PO）,. antibacteria performance （Ua）, lysozyme （ UL ）, superoxide dismutase （SOD）, alkaline phosphatase （AKP） and acic phosphatase （ACP） were measured. The results indicated that the effects of dietary VC on the activities of PO, Ua, UL, SOD, AKP and ACP in the tissues and organs of E. sinensis were significant difference （ P 〈 0.05 or P 〈0.01）.The activities of PO, Ua, UL, AKP and ACP in the serum, muscle, hepatopancrea and ovary of E. sinensis were significantly enhanced with Vc supplement increasing in the range from mg. （100g）^-1 diet to 100（ mg·（100g）^-1 diet （P 〈 0.05 or P 〈 0.01）. However, the activities of AKP were declined at 1500 mg·（100g） -1 diet Vc. The activities of SOD in the serum, muscle, hepatopancrea and ovary of E. sinensis wet significantly lowered with Vc supplementation. Vc could promoting non-special immunity, improve anima health, enhancing general metabolism of E. sinensis, and optimum supplementation of Vc to increase non specific immunity in E. sinensis was 500- 1000 mg·（lOOg）^- 1 diet Vc. The study also revealed the relationshi of the activities of enzymes and non-special immunity of E. sinensis.

关 键 词：中华绒螯蟹 维生素C 非特异性免疫 

分 类 号：Q591.6[生物学—生物化学] S963.12[农业科学—水产养殖]