新城疫病毒和阿霉素对肝癌细胞结构的影响  

Effects of Newcastle Disease Virus and Adriamycin on Surface Structure and Actin of Hepatocellular Carcinoma Cell Lines SMMC-7721

作　　者：赵铁军[1] 田伏洲[2] 李卫[1] 鲍传志 陈涛[2] 尹志良[2] 

机构地区：[1]成都军区总医院市内部外科,成都610017 [2]成都军区总医院普通外科,成都610083

出　　处：《中国普外基础与临床杂志》2008年第4期240-244,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：成都军区卫生科研基金资助(编号:99A007)~~

摘　　要：目的研究新城疫病毒(NDV)和阿霉素(ADM)对肝癌SMMC-7721细胞表面结构及肌动蛋白的影响。方法将肝癌SMMC-7721细胞分为2组,向其中一组加入NDV(NDV组),另一组加入ADM(ADM组)。设8、16、24、36和48 h 5个时相点进行培养。用免疫荧光法检测细胞肌动蛋白和Ca2+,用免疫组化法检测CD44,用流式细胞仪检测细胞间黏附分子-1(ICAM-1),用扫描电镜观察细胞表面结构改变。结果随着NDV和ADM作用时间的延长,细胞缩小、变圆;肌动蛋白断裂,排列紊乱,2组肌动蛋白平均荧光强度均降低,NDV组较ADM组下降更明显,8 h时2组间差异无统计学意义,而于16、24、36和48 h时2组间差异有统计学意义(36 h时P<0.01,其余时相P<0.05);2组癌细胞内Ca2+浓度逐渐增高,NDV组增高幅度明显大于ADM组,在8 h和16 h时差异虽无统计学意义,但已有差异的趋势,于24、36和48 h时差异有统计学意义(分别P<0.01,P<0.05和P<0.01);2组癌细胞表达的CD44逐渐减弱;2组ICAM-1平均荧光强度逐渐升高,至36 h时达峰值,2组间差异无统计学意义。上述各指标同一组内各时相间差异均有统计学意义(P<0.05)。结论NDV和ADM均可使癌细胞变性、破裂,NDV较ADM效果更强。Objective To compare the effects of newcastle disease virus （NDV） and adriamycin （ADM） on surface structure and actin of hepatocellular carcinoma cell lines SMMC-7721. Methods SMMC-7721 carcinoma cell lines were divided into 2 groups. NDV was added into one group, while ADM was added into the other group. The cells were then cultured at 5 time phases （8, 16, 24, 36 and 48 h）. Intracellular actin and Ca^2＋ were examined by using immunofluorescence method. CD44 and intercellular adhesion molecule-1 （ICAM-1） were detected by using immunochemical method and flow cytometry, respectively. The change of.cellular surface structure was observed by scan electron microscope. Results Cells gradually contracted and turned round over time. It was observed that actin was segmented and cells alignment became disordered. The mean fluorescence intensity of actin decreased in both groups, but it was obvious in NDV group. There were significant differences of fluorescence intensity between 2 groups at the phases of 16 h （P〈0.05）, 24 h （P〈0.05）, 36 h （P〈0.01） and 48 h （P〈0.05）, except the one after 8 h. Intracellular Ca^2＋ concentration increased gradually in both groups, and the amplifications in NDV group were significantly higher at the phases of 24 h, 36 h and 48 h than those in ADM group （P〈0.01, P〈0.05 and P〈0. 01, respectively）. There were also differences at 8 and 16 h, but there were no statistical significance. The expression of CD44 in cells decreased. The mean fluorescence intensity of ICAM-1 raised gradually, and then came to peaking at 36 h, but there was no significant difference between two groups. All the above indices between different phases in the same group showed significant differences （P〈0.05）. Conclusion Both NDV and ADM could make tumor cells degenerate and rupture, but the effect of NDV is more intensive. It could increase the fragility of cells and hasten the process of cell rupture. Disintegrated cancer cell and changes of adhesion molecule could

关 键 词：新城疫病毒 阿霉素 肝癌细胞 肌动蛋白 CA^2+ CD44 细胞间黏附分子-1 表面结构 

分 类 号：R735.7[医药卫生—肿瘤]