人乳腺癌原代细胞和细胞系中肿瘤干细胞的比较  

Compare Cancer Stem Cells in the Human Breast Cancer Cell Line with Those in the Primary Cells

作　　者：李治 刘春萍[1] 贺艳丽[1] 田元[1] 黄韬[1] 

机构地区：[1]华中科技大学协和医院乳腺甲状腺外科,武汉430022

出　　处：《中国医科大学学报》2008年第2期155-157,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30571798);湖北省"十一五"重大科技攻关项目(2006AA301A05)

摘　　要：目的比较人乳腺癌原代细胞以及人乳腺癌细胞系MCF-7、MDA-MB-231S中乳腺癌干细胞的含量和细胞表型。方法采用流式细胞技术对人乳腺癌原代细胞以及MCF-7、MDA-MB-231S进行检测和分选,并测定不同细胞亚群在裸鼠体内重建肿瘤的能力。结果人乳腺癌原代细胞以及MCF-7、MDA-MB-231S细胞的CD44和CD24表达与分布不同;和人乳腺癌原代细胞相比,MCF-7、MDA-MB-231S细胞中乳腺癌干细胞的含量更高(P<0.01)。结论和人乳腺癌原代细胞相比,人乳腺癌细胞系中含有更高比例的乳腺癌干细胞,并且细胞表型也发生了改变。Objective To compare the content and phenotype of cancer stem cells in the MCF-7, MDA-MB-231S human breast cancer cell line with those in the human breast cancer primary cells. Methods Different subpopulation cells were detected and separated from MCF-7, MDA-MB-231S and human breast cancer primary cells by flow cytometry,and their ability to reconstruct tumor was determined in vivo. Results The expression and distribution of CD44 and CD24 in MCF-7,MDA-MB-231S and human breast cancer primary ceils were different. Compared with human breast cancer primary cells,the proportion of cancer stem ceils in MCF-7 and MDA-MB-231S ceils was higher （P 〈 0.01 ）. Conclusion Compared with human breast cancer primary ceils, there am more breast cancer stem cells in the human breast cancer cell line and the phenotype has been changed.

关 键 词：干细胞 原代细胞 乳腺癌 细胞表型 

分 类 号：R735.7[医药卫生—肿瘤]