LPS介导的人牙周膜成纤维细胞损伤过程中TRAF-6基因的表达  

Expression of TRAF-6 gene in LPS mediated damage of human periodontal ligament fibroblasts

作　　者：徐利东[1] 李卫星[1] 李霞[1] 任娟[1] 程珏[1] 

机构地区：[1]山西医科大学口腔医学院口外科,太原030001

出　　处：《山西医科大学学报》2008年第4期333-335,共3页Journal of Shanxi Medical University

基　　金：山西省青年科研基金资助项目(2006021041)

摘　　要：目的观察不同浓度LPS干预下,肿瘤坏死因子受体相关因子-6(TRAF-6)在人牙周膜成纤维细胞中基因表达和定位情况。方法体外分离培养人牙周膜成纤维细胞并鉴定,采用逆转录多聚酶链反应法(RT-PCR)和计算机图像分析方法,动态观察TRAF-6在不同浓度LPS干预下的表达和定位特点。结果随LPS干预浓度由0.1μg/ml增加到100μg/ml,TRAF-6基因条带表达为先逐渐增高然后又降低的趋势。结论本实验说明TRAF-6在LPS引发的牙周膜成纤维细胞炎症损伤中可能发挥重要的作用,TRAF-6可能是一种影响牙周膜成纤维细胞分化的胞内信号转导分子。Objective To investigate the gene expression and the localization of tumor necrosis factor receptor-associated factor-6 （TRAF-6） in human periodontal ligament fibroblasts （HPLFs）after the intervention with different concentrations of lipopolysaccharide. Methods Human periodontal ligament cells were isolated and identified. By reverse transcription polymerase chain reaction（RT-PCR）and computer image analysis technique, the expression and localization of TRAF-6 were observed. Results With the increase of concentration of LPS from 0.1μg/ml to 100μg/ml,the expression of TRAF-6 gene increased gradually and then decreased. Conclusion The result indicates that TRAF-6 may play an important role in the developmentof experimental periodontitis. It may be a signal transducer that influence the proliferation of HPLFs.

关 键 词：人牙周膜成纤维细胞 脂多糖 肿瘤坏死因子受体相关因子-6 逆转录多聚酶链反应法 

分 类 号：R781.4[医药卫生—口腔医学]