检测癌症患者外周血淋巴细胞评估化疗药物敏感性的研究  

Study on MTT Prepare to Make to Examine the Plank for Chemosensitivity in Peripheral Blood Iymphocyte with Cancer Patients

作　　者：霍红旗[1] 刘爱民[1] 李鹏[1] 王海东[1] 

机构地区：[1]河北省邯郸市中心医院核医学科,河北邯郸056001

出　　处：《现代检验医学杂志》2008年第2期33-35,共3页Journal of Modern Laboratory Medicine

摘　　要：目的基于众多文献报道四甲基偶氮唑蓝(MTT)法化疗药敏测定中癌细胞抑制率与外周血淋巴细胞抑制率呈正相关性,探讨MTT法预制检测板在检测癌患者外周血淋巴细胞药物敏感性中的价值,以指导临床化疗用药。方法应用泰伦公司生产的预制检测板,对75例癌患者外周血淋巴细胞进行20种药物的药敏检测,操作方法按说明书进行。结果39例肺癌患者外周血淋巴细胞对化疗药物敏感性,由高到低前5名依次为:卡氮芥(BCNU),紫杉醇(PTX),阿霉素(ADM),长春瑞滨(NVB)和表阿霉素(e-ADM);25例乳腺癌患者血淋巴细胞对化疗药物敏感性,前5名依次为BCNU,PTX,NVB,ADM和米托蒽醌(MIT);11例胃癌患者血淋巴细胞对化疗药物敏感性,前5名依次为PTX,ADM,BCNU,eADM和阿糖胞苷(Ara-C)。结论该MTT法预制检测板适用于癌患者药物敏感性试验,对临床癌患者个体化化疗有重要指导意义。Objective Based on lots of MTT chemosensitivity assay demonstrating that the inhibited rates between cancer cells and peripheral blood lymphocytes showed positive correlation,to explore the value of the MTT prepare to make to examine the plank in peripheral blood lymphocytes for three kind of cancer patients. Methods Using Talent MTT prepare to make to examine the plank, 75 peripheral blood lymphocyte samples from three kind of cancer patients were analyzed by chemosensitivity assay with 20 anticancer drugs. Results To 39 lung cancer patients,the 5 anticancer drugs had higher sensitivity and were BCNU,PTX,ADM,NVB and e-ADM ;To 25 breast cancer patients ,the 5 anticancer drugs were BCNU,PTX,NVB,ADM and MIT;To 11 gastric cancer patients,they were PTX,BCNU,ADM,e-ADM and Ara-C. Conclusion The MTT prepare to make to examine the plank is applicable for the chemosensitivity assessment of peripheral blood lymphocytes with the cancer patients and is of important reference value to choose chemotherapeutic drugs in clinic.

关 键 词：抗肿瘤 MTT法预制检测板 化疗药物敏感试验 淋巴细胞 

分 类 号：R73-361[医药卫生—肿瘤]