乙醇诱导大鼠肝损伤的代谢组学和茵陈蒿汤的干预研究  

Studies on the metabonomics of rat liver injury induced by ethanol and interfering effects of Yin Chen Hao Tang

作　　者：王喜军[1] 刘莲[1] 孙晖[1] 孙文军[1] 吕海涛[1] 

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《中国药理学通报》2008年第4期452-457,共6页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(No2005CB523406);国家自然科学基金重大研究计划资助项目(No90709019)

摘　　要：目的通过乙醇诱导大鼠肝损伤的代谢组学研究,确定能够表征酒精性肝损伤发生、发展和恢复状态的生物标记物,并基于此标记物的代谢轨迹变化阐明茵陈蒿汤的保肝作用。方法基于代谢组学理论,以UPLC-ESI-Qtof/MS为核心技术,以主成分分析方法为数据解析手段,研究空白大鼠、乙醇诱导肝损伤大鼠和茵陈蒿汤干预大鼠尿液中代谢物组时量的轨迹变化和对应关系。结果初步确定表征4个能够表征酒精性肝损伤发生、发展和恢复状态的生物标记物内源性生物标记物,且茵陈蒿汤对4个内源性生物标记物代谢轨迹的扰动具明显的回调作用。结论代谢组学方法成功用于乙醇诱导的大鼠肝损伤研究,并且从生物体内代谢组层面确定茵陈蒿汤具有较好的保肝作用。Aim To determine potential biomarkers contributed to occurrence, development and recovery of ethanol-induced liver injury in rat and elucidate hepatoprotective effect of Yin Chen Hao Tang based on metabonomic investigation. Methods A UPLC-QTOF/MS based metabonomic investigating trajectory urinary metabolome of chang method was developed for e and inter-relationship of rats with different treatments. were determinedwhich contributed to occurrence, development and recovery of ethanol-induced liver injury in rat, and Yin Chen Hao Tang could significantly recover trajectory change in disorder. Conclusion The developed method was successfully applied to investigate ethanol-induced liver injury in rat, and also hepatoprotective effect of Yin Chen Hao Tang was elucidated.

关 键 词：UPLC-Q-TOF/MS 代谢物组学 生物标记物 茵陈蒿 汤 肝损伤 

分 类 号：R-332[医药卫生] R285.5