龙眼参多糖对SAMP8鼠脑组织痴呆相关基因表达的影响  

Effect of LYS polysaccharides on dementia-related gene expressions in SAMP8 mouse brain

作　　者：黄忠仕[1] 焦杨[1] 张士军[1] 蒋伟哲[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,广西南宁530021

出　　处：《中国药理学通报》2008年第4期509-512,共4页Chinese Pharmacological Bulletin

基　　金：广西科学研究与技术开发计划资助项目(No桂科攻0630002-2A)

摘　　要：目的观察龙眼参(LYS)多糖对SAMP8(快速老化小白鼠)鼠脑内APP、PS1、PS2、ApoE基因表达的影响。方法选用6mon龄的SAMP8鼠50只,随机分为5组,每组10只:SAMP8对照组、阳性药石杉碱甲对照组、多糖低、中、高剂量组,另选用6monSAMR1(正常老化小白鼠)鼠10只作正常对照组。各组分别灌胃相应药物40d后,采用半定量反转录聚合酶链反应(RT-PCR)来测定SAMP8鼠脑内APP、PS1、PS2、ApoE mRNA含量。结果龙眼参多糖低、中、高剂量能降低脑内APP、PS1、PS2 mRNA含量并呈现一定的量效关系,对ApoE mRNA含量的影响不明显。结论龙眼参多糖能下调SAMP8脑内APP、PS1、PS2基因的表达。Aim To observe the effects of LYS polysaccharides on expressions of APP, PS1, PS2 and ApoE in SAMP8 mouse brain. Methods 50 6-monthold SAMP8 mice were used and divided randomly into 5 groups： SAMP8 untreated control group, huperzine A control group, low-, mid- and high-dose groups of polysaccharides, with 10 mice in each group. 10 6- month-old SAMR1 mice were used as normal control. After each group was treated by corresponding drug for 40 days continuously, the mRNA contents of APP, PS1, PS2 and ApoE in brain tissue were assayed by re-verse transcription polymerase chain reaction. Results The low-, mid- and high-dose groups of polysaccharides could reduce the mRNA contents of APP, PS1 and PS2, which showed a dose-effect relationship in some degrees. But it could not affect the content of ApoE. Conclusion LYS polysaccharides could inhibit the expressions of APP, PS1, and PS2 in SAMP8 mouse brain.

关 键 词：龙眼参多糖 痴呆 基因表达 APP PS1 PS2 APOE 

分 类 号：R-332[医药卫生] R284.1