枕大池注入促红细胞生成素在兔蛛网膜下腔出血的脑保护作用  

The neuroprotective effect by single intracisternal injection of erythropoietin on a rabbit model of subarachnoid hemorrhage

作　　者：尹泓[1] 薛欣盛[2] 马永丰[1] 张文胜[2] 易明亮[2] 廖大清[2] 刘进[2] 

机构地区：[1]兰州大学附属第二医院麻醉科,甘肃兰州730000 [2]四川大学华西医院麻醉与危重急救研究室,四川成都610041

出　　处：《中国药理学通报》2008年第4期547-551,共5页Chinese Pharmacological Bulletin

摘　　要：目的研究枕大池单次注入促红细胞生成素(EPO)对蛛网膜下腔出血后脑血管痉挛和神经细胞损伤的影响。方法30只新西兰白兔随机分为3组,假手术组(Sham组)、蛛网膜下腔出血组(SAH组)和EPO组(n=10)。Sham组穿刺,其它两组行枕大池一次穿刺注血造模。30min后假手术组和出血组从枕大池注入生理盐水(0·10ml·kg-1),EPO组注入EPO(200IU·kg-1,0·10ml·kg-1)。所有动物在造模后48h处死,使用CAST体视学图像分析系统测量分析并比较不同组间基底动脉管腔面积、收缩系数以及海马CA1区正常神经元密度。结果与SAH组相比,EPO组食欲明显改善(P<0·05),体重增加(P<0·05),基底动脉管腔面积明显增加(P<0·01),收缩系数减小(P<0·01),海马CA1神经元密度值也增加(P<0·05)。结论枕大池单次小剂量注入EPO可以预防SAH后脑血管痉挛,减轻脑神经细胞损伤。Aim To investigate the neuroprotective effect by a single intracisternal bolus injection of erythropoietin on rabbits subjected to subarachnoid hemorrhage. Methods Thirty male New Zealand adult white rabbits weighing 2. 0 - 2. 5 kg were randomized to threegroups（n = 10 in each group） ： Sham group, SAH group and erythropoietin （EPO） group. SAH model of single-hemorrhage was made by injecting autologous arterial blood （ 1.0 ml·kg^-1 ） into the cistema magna in SAH group and EPO group rabbits, while animals in sham group received an injection of normal saline （ 1.0 ml·kg^-1 ）. At thirty minutes after SAH, rabbits of sham group and SAH group were injected normal saline （0. 1 ml·kg^-1 ） into the cisterna magna, while EPO group received an injection of EPO （200 IU ml·kg^-1 , 0. 1 ml ml·kg^-1 ）. All animals were killed at 48 hours after SAH. The cross section areas of basilar arteries, corrugation coefficient （ CC ） of basilar arteries, and hippocampus normal neuron density of CA1 were measured by CAST system （computerized assist stereology technology）. Results Compared with those in SAH group, the cross-sectional area of basilar arteries in EPO group was significantly greater（ P 〈 0. 05 ） ; CC in EPO group was significantly lower（ P 〈 0. 05 ） and CA1 normal neuron density of hippocampus was significantly higher（P 〈 0. 05 ） in EPO group. Conclusions A single intracisternal bolus of erythropoietin could prevent SAH-induced CVS and neuron injury in a rabbit model of SAH.

关 键 词：促红细胞生成素 蛛网膜下腔出血 血管痉挛 海马 

分 类 号：R-332[医药卫生] R322.81