大鼠视上核和室旁核神经元钾离子氯离子共转运体2表达探讨  

Absence expression of neuronal specific potassium chloride ion cotransporter 2 in supraoptic and paraventricular nuclei

作　　者：王德广[1] 张静[2] 陈菲菲[2] 郑殿杰[2] 陈幽婷[3] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,江苏徐州221002 [2]徐州医学院神经生物学教研室 [3]徐州医学院解剖学教研室

出　　处：《徐州医学院学报》2008年第4期215-217,共3页Acta Academiae Medicinae Xuzhou

基　　金：江苏省教育厅资助课题(06KJD310188);江苏省麻醉医学研究所开放课题(卫WK200601)

摘　　要：目的观察神经元特异性标记物钾离子氯离子共转运体2在成年大鼠下丘脑视上核和室旁核的表达。方法成年SD大鼠,4%多聚甲醛灌注固定,取脑,冰冻切片,下丘脑部进行钾离子氯离子共转运体2和神经细胞核抗原免疫荧光染色,激光共聚焦显微镜观察。结果钾离子氯离子共转运体2在视上核和室旁核神经元中表达很弱。结论下丘脑视上核和室旁核部分神经元缺少神经特异性的钾离子氯离子共转运体2表达。Objective To observe the neuronal specific expression of potassium chloride ion cotrausporter 2 in adult rat supraoptic and paraventricular nuclei. Methods The adult SD rats were sacrificed and fixecl with 4% paraformaldehyde to remove the brain for frozen - sectioning. The expression of potassium chloride ion cotransporter 2 and NeuN revealed by immunofluorescence were studied in the photos taken under laser＂confocal microscope. Results The expression of potassium chloride ion cotransporter 2 was absent from the supraoptic and paraventricular nuclei. Conclusion The neuronal specific potassium chloride ion cotransporter 2 is absent in the supraoptic and paraventricular nuclei.

关 键 词：下丘脑 视上核 室旁核 钾离子氯离子共转运体2 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]