H22移植性肝癌高强度聚焦超声治疗后的免疫变化  

Impact of High Intensity Focused Ultrasound Therapy on Immunity in Transplanted H22 Hepatic Carcinoma

作　　者：谢勋鹏[1] 冉立峰[2] 伍烽[3] 王智彪[3] 范艳敏[3] 赵颉[3] 

机构地区：[1]南通大学附属第三医院,江苏南通226006 [2]重庆医科大学附属第二医院,重庆400010 [3]重庆医科大学医学超声工程研究所,重庆400016

出　　处：《肿瘤学杂志》2008年第4期254-256,共3页Journal of Chinese Oncology

摘　　要：[目的]探讨高强度聚焦超声(HIFU)治疗H22移植性肝癌后的细胞免疫学变化。[方法]将90只C57BL/6J小鼠随机为3组,HIFU治疗组和HIFU假照组于建立H22移植性肝癌模型1周后接受HIFU治疗和假照,正常对照组小鼠不接种肿瘤也不接受HIFU照射,治疗后2周流式细胞术测定各组小鼠外周血T淋巴细胞亚群的变化。[结果]HIFU治疗组治疗后肿瘤完全消褪,而HIFU假照组无肿瘤消褪。与假照组相比,HIFU治疗组和正常对照组小鼠CD3+、CD4+T淋巴细胞,CD4+/CD8+比值均明显升高(P<0.01),CD8+T淋巴细胞均明显降低(P<0.05);而HIFU治疗组与正常对照组比较,各指标均无明显差异。[结论]HIFU治疗H22移植性肝癌后,肿瘤消褪,且免疫功能明显增强。HIFU治疗可提高机体抗肿瘤的免疫作用。[Porpuse] To investigate the impact of high intensity focused ultrasound （HIFU） therapy on immunity in H22 transplanted hepatic carcinoma. [ Methods ] Ninety C57 BL/6] mice were randomly divided into three groups. One week after tumor implantation, the mice underwent HIFU ablation or HIFU sham ablation for the implanted tumor. Those in control group were normal mice, without either tumor implantation or HIFU treatment. Two weeks after HIFU, peripheral blood of each mouse was collected to detect T lymphocyte and subset by fluorescein activated cell sortor （FACS）. [Results] Two weeks after HIFU, the tumor mass were completely regressive in all HIFU treated mice; none regression in sham-HIFU treated mice. Compared to the values in either sham-HIFU group, there were a significant increase of CD3^＋, CD4^＋ level and CD4^＋/CD8^＋ ratios, and a significant decrease of CD8^＋ level in HIFU treated group and control group. But compared with the values in either HIFU treated group and control group, there were no significant difference of CD3^＋, CD4^＋, CD8^＋ level and CD4^＋/ CD8^＋ ratios. [Conclusion] The tumor mass can be regressive and the immunity improves after HIFU treatment for implanted H22 hepatic carcinoma. HIFU improves the anti-tumor immunity.

关 键 词：高强度聚焦超声 肝肿瘤 免疫学 

分 类 号：R730.5[医药卫生—肿瘤]