南方鲶胚胎细胞培养及其细胞周期特性检测  

SUBCULTURE AND OBSERVATION OF CELL CYCLE CHARACTERISTICS OF EMBRYONIC CELL FROM SILUROIDEI SOLDATOV MERIDIONALIS

作　　者：洪锡钧[1] 

机构地区：[1]西南师范大学生命科学系,重庆630715

出　　处：《水产学报》1997年第3期240-245,共6页Journal of Fisheries of China

摘　　要：南方鲶晚期胚胎细胞经原代和传代培养40次，历时36个月建立了上皮型细胞系（SM）。该细胞系表现低世代生长缓慢，每代时间随代龄增加而减少，经EMF测定：G1期细胞占70．1％～85．6％；S期细胞占12．2％～25．3％，G2+M期细胞占1.6％～4．6％，由30代到40代G2+M细胞增加了一倍多，SM细胞能在1～4℃冰箱长期存活并可保持繁殖能力达13个月。The paper deals with the fish SM cell line which originally came form the culture of late embronic cells of S. sodatovi meridionalis Chen.The cell line has been established through 40 subculture passage over a period of 36 months.SM cell generation time (days)decreased with increasing subculture time.The subculture substratum was RPMI 1640 supplemented with moderate antibiotics 20% newborn calf serum and incubated at temperature 28～29℃, pH 6.5～ 7.2. The results of flow microfluoronetry(FMF) showed the percentage of cells at G1 phase is 70. 1% ～85.6%; S phase is 12.2%～25.3% and G2+ M phase are 1.6% ～ 4.6% .With the increase of passages the percentage of the cells at G2 + M phase got higher. In laboratory SM cells were cultured below the temperature of 1℃ to 4℃ they could survive for 13 months.

关 键 词：南方鲶 胚胎细胞 传代培养 细胞周期 

分 类 号：Q959.4[生物学—动物学] Q954.4